HNDS (BLUE), HPTS (GREEN), ethanol, sodium hypochlorite solution (10–15%), calcium chloride dihydrate, anhydrous sodium carbonate, anhydrous sodium bicarbonate, taurine, phenol, potassium carbonate, anhydrous pyridine, anhydrous N,N-dimethylformamide and ethylenediamine tetraacetic acid disodium salt (Na2.EDTA) were used as purchased from Sigma-Aldrich. Sulphuric acid was used as purchased from Fluka. 1,6,7,12-tetrachloroperylene-3,4,9,10-tetracarboxylic acid dianhydride was used as purchased from Santa Cruz Biotechnology. Deionized (DI) water was used as obtained from in-house Millipore Q system (<2 p.p.m. total organic content (TOC) and 18.2 MΩ).
Ethylenediamine tetraacetic acid disodium salt na2 edta
Manufactured by Merck Group
Sourced in United States
Ethylenediamine tetraacetic acid disodium salt (Na2.EDTA) is a chemical compound commonly used as a chelating agent in various laboratory applications. It is a crystalline, water-soluble powder that forms stable complexes with metal ions. Na2.EDTA is widely used in analytical chemistry, biochemistry, and biotechnology for the purification, stabilization, and analysis of various biomolecules and metal-containing compounds.
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Lab products found in correlation
Anhydrous sodium carbonate, by Merck Group (1 mentions)
Anhydrous pyridine, by Merck Group (1 mentions)
Anhydrous n n dimethylformamide, by Merck Group (1 mentions)
Phenol, by Merck Group (1 mentions)
Taurine, by Merck Group (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Sodium hypochlorite solution, by Merck Group (1 mentions)
Potassium carbonate, by Merck Group (1 mentions)
Methylene blue, by Thermo Fisher Scientific (1 mentions)
Ultrapure water, by Yamato Scientific (1 mentions)
Parabenzoquinone pbq, by Merck Group (1 mentions)
Methyl orange, by Thermo Fisher Scientific (1 mentions)
Ampicillin sodium salt, by Thermo Fisher Scientific (1 mentions)
Restriction endonuclease dpni, by Thermo Fisher Scientific (1 mentions)
Milli q purification system, by Merck Group (1 mentions)
Imidazole, by Merck Group (1 mentions)
Px459 plasmid, by Addgene (1 mentions)
Turbofect cationic polymerbased transfection reagent, by Thermo Fisher Scientific (1 mentions)
Genejet dna spin column based purification kit, by Thermo Fisher Scientific (1 mentions)
Endotoxin binding reagent, by Thermo Fisher Scientific (1 mentions)
3 protocols using ethylenediamine tetraacetic acid disodium salt na2 edta
1
Synthesis of Perylene Tetracarboxylic Dyes
2
Synthesis and Characterization of Transition Metal Oxides
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Titanium(iv ) isopropoxide (TTIP, ACROS ORGANICS, CHINA), isopropanol (≥99.8% (GC), Honeywell|Riedel-de Haën™, France), sodium tungstate oxide dihydrate (Na2WO4·2H2O, 99.0–101.0%, Thermo Fisher Scientific.), ethanol (≥99.9%, Honeywell|Fluka™, Germany), nitric acid (65% w/w), hydrochloric acid (37%, reagent grade), ethylene glycol (99.5%, ACROS ORGANICS), methylene blue (ACROS ORGANICS, INDIA), and methyl orange (ACROS ORGANICS, INDIA) were used without further purification. Para-benzoquinone (p-BQ) and ethylenediaminetetraacetic acid disodium salt (Na2-EDTA) were purchased from Sigma-Aldrich and Fisher Chemical, respectively. Ultrapure water (resistivity of 18.2 mΩ cm, Yamato, Japan) was used throughout the experiments.
3
Production and Purification of SMALPs
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Commercial styrene-maleic acid (SMAc) co-polymers X25 (Xiran SL 25010 P20, lot number from EtOH before use. THF was purified and dried according to the reported procedures and stored under nitrogen [22] (link). MAn was used as received.
Buffers for the preparation of SMALPs were prepared in ultrapure water treated with a Milli-Q purification system (Merck, USA). Potassium phosphate monobasic (KH2PO4), potassium phosphate dibasic (K2HPO4), sodium chloride (NaCl), tris(hydroxymethyl)aminomethane (Tris), hydrochloric acid (HCl), glycerol, sucrose, calcium chloride (CaCl2) dihydrate, protease inhibitor cocktail tablets (SigmaFAST), ethylenediaminetetraacetic acid disodium salt (Na2EDTA), nickel(II) chloride (NiCl2) hexahydrate, imidazole and polyethylene glycol sorbitan monolaurate (Tween-20) were purchased from Sigma-Aldrich (Merck, USA).
The pX459 plasmid was purchased from Addgene (USA). Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin solution (5000 U/mL penicillin, 5000 µg/mL streptomycin), ampicillin sodium salt, the restriction endonuclease DpnI, the Turbofect cationic polymerbased transfection reagent, the GeneJet DNA spin column-based purification kit and the endotoxin binding reagent were purchased from ThermoFisher Scientific (USA). Puromycin solution (10 mg/mL) was purchased from Invivogen (France).
Buffers for the preparation of SMALPs were prepared in ultrapure water treated with a Milli-Q purification system (Merck, USA). Potassium phosphate monobasic (KH2PO4), potassium phosphate dibasic (K2HPO4), sodium chloride (NaCl), tris(hydroxymethyl)aminomethane (Tris), hydrochloric acid (HCl), glycerol, sucrose, calcium chloride (CaCl2) dihydrate, protease inhibitor cocktail tablets (SigmaFAST), ethylenediaminetetraacetic acid disodium salt (Na2EDTA), nickel(II) chloride (NiCl2) hexahydrate, imidazole and polyethylene glycol sorbitan monolaurate (Tween-20) were purchased from Sigma-Aldrich (Merck, USA).
The pX459 plasmid was purchased from Addgene (USA). Dulbecco's Modified Eagle Medium (DMEM), fetal bovine serum (FBS), penicillin-streptomycin solution (5000 U/mL penicillin, 5000 µg/mL streptomycin), ampicillin sodium salt, the restriction endonuclease DpnI, the Turbofect cationic polymerbased transfection reagent, the GeneJet DNA spin column-based purification kit and the endotoxin binding reagent were purchased from ThermoFisher Scientific (USA). Puromycin solution (10 mg/mL) was purchased from Invivogen (France).
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