18F-BCPP-EF (58.5–66.7 MBq, 2.7–3.7 nmol/kg animal, iv) was injected 3 h and 24 h after the QA injection. The PET scans were conducted with a high-resolution animal PET scanner (SHR-38000; Hamamatsu Photonics, Hamamatsu, Japan) under isoflurane anaesthesia. Dynamic images and summation images of 18F-BCPP-EF, from 0 to 90 min after the injection, were reconstructed and used to create standardised uptake value (SUV) images. The body temperatures of the rats were monitored and maintained using a heating pad during PET measurement. Volumes of interest (VOIs) were placed on PET images in the striatum, prefrontal cortex, frontal cortex, parietal cortex, hippocampus, thalamus, and cerebellum, with the aid of Waxholm Space Atlas of the Sprague Dawley Rat Brain (the INCF Software Center, https://www.nitrc.org/projects/whs-sd-atlas ) and rat brain atlas [34 ].
Shr 38000
Manufactured by Hamamatsu Photonics
Sourced in Japan
The SHR-38000 is a high-speed, high-resolution photomultiplier tube (PMT) developed by Hamamatsu Photonics. It offers a large effective photocathode area and a high gain, making it suitable for a variety of applications that require sensitive light detection.
Automatically generated - may contain errors
Lab products found in correlation
4 protocols using shr 38000
1
In Vivo Imaging of Neuroinflammation in Rat Brain
2
Multimodal Neuroimaging of Primate Brain
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A
structural T1 weighted magnetic resonance
imaging (MRI) scan (Signa Excite HDxT 3.0T, GE Healthcare, Milwaukee,
WI, USA) was made before the first PET scan. All animals underwent
91-min dynamic PET scan (SHR-38000, Hamamatsu Photonics, Shizuoka,
Japan) with arterial blood sampling. Animals were anesthetized (2.5%
sevoflurane) during their transport but remained awake during the
scans with their head immobilized using a fixation device. The monkeys
were positioned in the camera in a sitting position, with stereotactic
coordinates aligned parallel to the orbitomeatal plane.
A transmission
scan was performed before the acquisition of the PET data using a
rotating 68Ge/68Ga rod source (60 min), and
its information was used for attenuation and scatter correction of
the PET images. Animals were injected with [18F]MC225 (684
± 64 MBq) at the start of the emission scan, via the saphenous
vein over a period of 30 s as a single bolus.
PET images were
reconstructed using filtered back projection with
a Hanning filter of 4.5 mm and were composed of 49 frames (6 ×
10, 6 × 30, 12 × 60, and 25 × 180 s).
structural T1 weighted magnetic resonance
imaging (MRI) scan (Signa Excite HDxT 3.0T, GE Healthcare, Milwaukee,
WI, USA) was made before the first PET scan. All animals underwent
91-min dynamic PET scan (SHR-38000, Hamamatsu Photonics, Shizuoka,
Japan) with arterial blood sampling. Animals were anesthetized (2.5%
sevoflurane) during their transport but remained awake during the
scans with their head immobilized using a fixation device. The monkeys
were positioned in the camera in a sitting position, with stereotactic
coordinates aligned parallel to the orbitomeatal plane.
A transmission
scan was performed before the acquisition of the PET data using a
rotating 68Ge/68Ga rod source (60 min), and
its information was used for attenuation and scatter correction of
the PET images. Animals were injected with [18F]MC225 (684
± 64 MBq) at the start of the emission scan, via the saphenous
vein over a period of 30 s as a single bolus.
PET images were
reconstructed using filtered back projection with
a Hanning filter of 4.5 mm and were composed of 49 frames (6 ×
10, 6 × 30, 12 × 60, and 25 × 180 s).
3
Primate PET Imaging with (R)-[11C]Verapamil
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The same acquisition protocol was used
in a previous study.37 (link) Briefly, 1 week
before the first PET scan, a
brain T1-weighted magnetic resonance imaging
(MRI) scan of the animal was made (Signa Excite HDTx 3.0T, GE Healthcare).38 (link) Brain PET scans were acquired using a high-resolution
animal PET scanner (SHR-38000, Hamamatsu Photonics). All nonhuman
primates underwent dynamic PET scans with arterial blood sampling.
Monkeys were anesthetized (2.5% sevoflurane) during the arterial cannulation
and their transport, but they were awake during the scans and the
head was immobilized using a fixation device. The animals were positioned
in the camera in a sitting position with stereotactic coordinates
aligned paralleled to the orbitomeatal plane.
Prior to tracer
injection, a rotating 68Ge/68Ga rod source was
used to perform the transmission scan (60 min), and its information
was used for attenuation correction of the PET images. Next, animals
were injected with (R)-[11C]verapamil
(more information inTable 2 ) at the start of the emission scan (91 min) via the saphenous vein over a period of 30 s as a single bolus.
PET images were reconstructed using the filtered back projection
method with a Hanning filter of 4.5 mm in SHR-38000 Reconstruction
software (Hamamatsu Photonics) and were composed of 49 frames (6 ×
10, 6 × 30, 12 × 60, and 25 × 180 s).
in a previous study.37 (link) Briefly, 1 week
before the first PET scan, a
brain T1-weighted magnetic resonance imaging
(MRI) scan of the animal was made (Signa Excite HDTx 3.0T, GE Healthcare).38 (link) Brain PET scans were acquired using a high-resolution
animal PET scanner (SHR-38000, Hamamatsu Photonics). All nonhuman
primates underwent dynamic PET scans with arterial blood sampling.
Monkeys were anesthetized (2.5% sevoflurane) during the arterial cannulation
and their transport, but they were awake during the scans and the
head was immobilized using a fixation device. The animals were positioned
in the camera in a sitting position with stereotactic coordinates
aligned paralleled to the orbitomeatal plane.
Prior to tracer
injection, a rotating 68Ge/68Ga rod source was
used to perform the transmission scan (60 min), and its information
was used for attenuation correction of the PET images. Next, animals
were injected with (R)-[11C]verapamil
(more information in
PET images were reconstructed using the filtered back projection
method with a Hanning filter of 4.5 mm in SHR-38000 Reconstruction
software (Hamamatsu Photonics) and were composed of 49 frames (6 ×
10, 6 × 30, 12 × 60, and 25 × 180 s).
4
Brain MRI and PET Imaging in Animals
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First, a T1-weighted brain MRI scan of each subject was acquired (Signa Excite HDTx 3.0 T scanner, GE Healthcare). Afterward, animals underwent a 60-min transmission scan using a rotating 68Ge/68Ga rod source followed by a 91-min dynamic emission PET scan (SHR-38000, Hamamatsu Photonics) with arterial blood sampling. Animals were injected with (R)-[11C]verapamil (954.2 ± 45.6 MBq, with 99.3 ± 0.2% radiochemical purity and a molar activity (MA) higher than 20 GBq/μmol), or [18F]MC225 (684 ± 64 MBq, with a purity of 97.6 ± 1.0% and a MA higher than 36 GBq/μmol), as a single bolus (over 30 s) via the saphenous vein, at the start of the emission scan (for more information [35 , 36 (link)]).
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