HEK293 cells were maintained by DMEM (Thermo Fisher Scientific) supplemented with 10% Fetal Bovine Serum (Cytiva, Marlborough, MA, USA) and Antibiotic-Antimycotic Stock Solution (NACALAI TESQUE, Inc., Kyoto, Japan). For replating cells, Trypsin-EDTA solution (Merck, Darmstadt, Germany) was used. HUVECs were purchased from Kurabo industries (Osaka, Japan) and Takara. HUVECs were maintained in Endothelial Cell Basal Medium 2 supplemented with Endothelial Cell Growth Medium kits (C22211, C22111, Takara). For replating cells, the cells were washed once with Hepes-buffered saline (Hepes-BSS), trypsinized with Trypsin-EDTA (CC5012, Lonza, Basal, Switzerland) and neutralized with Hepes-BSS/10% FBS. The cells were centrifuged to eliminate Hepes-BSS/10% FBS and plated with the growth medium. The cells were used by passage 5.
Antibiotic antimycotic stock solution
Manufactured by Nacalai Tesque
Sourced in United States
Antibiotic-Antimycotic Stock Solution is a laboratory product that contains a mixture of antibiotics and antimycotics. It is commonly used to prevent bacterial and fungal contamination in cell culture applications.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Cytiva (2 mentions)
Trypsin edta, by Lonza (2 mentions)
Trypsin edta solution, by Merck Group (2 mentions)
Huvecs, by Takara Bio (2 mentions)
Hepes bss, by Lonza (2 mentions)
Endothelial cell growth medium kits, by Takara Bio (2 mentions)
2 protocols using antibiotic antimycotic stock solution
1
Cell Culture Protocols for HEK293 and HUVEC
2
Culturing HEK293 and HUVEC cells
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HEK293 cells were maintained by DMEM (Thermo Fisher) supplemented with 10% Fetal Bovine Serum (Cytiva, Marlborough, MA, USA) and Antibiotic-Antimycotic Stock Solution (NACALAI TESQUE, Inc., Kyoto, Japan). For replating cells, Trypsin-EDTA solution (Merck, Darmstadt, Germany) was used. HUVECs were purchased from Kurabo industries (Osaka, Japan) and Takara. HUVECs were maintained in Endothelial Cell Basal Medium 2 supplemented with Endothelial Cell Growth Medium kits (C22211, C22111, Takara). For replating cells, the cells were washed once with Hepes-buffered saline (Hepes-BSS), trypsinized with Trypsin-EDTA (CC5012, Lonza, Basal, Switzerland) and neutralized with Hepes-BSS/10% FBS. The cells were centrifuged to eliminate Hepes-BSS/10% FBS and plated with the growth medium. The cells were used by passage 5.
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