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14c sucrose

Manufactured by American Radiolabeled Chemicals
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14C-sucrose is a radiolabeled form of sucrose, a common disaccharide sugar. It contains a carbon-14 isotope, which allows for its detection and quantification in various applications. The core function of 14C-sucrose is to serve as a tracer or marker in scientific research and analysis.

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Lab products found in correlation

3h diazepam, by American Radiolabeled Chemicals (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Sequencing grade trypsin, by Thermo Fisher Scientific (1 mentions) Ultima gold scintillation cocktail, by PerkinElmer (1 mentions) 14c caffeine, by American Radiolabeled Chemicals (1 mentions) 3h digoxin, by American Radiolabeled Chemicals (1 mentions) Tmtpro 16plex label reagent set, by Thermo Fisher Scientific (1 mentions) Dulbecco s modified eagle medium dmem, by Merck Group (1 mentions) Bovine serum albumin (bsa), by Merck Group (1 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Ko143, by Bio-Techne (1 mentions) Mk571, by Merck Group (1 mentions) Psc833, by Bio-Techne (1 mentions)

3 protocols using 14c sucrose

1

Radioactive Probes for Cellular Assays

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All radioactive probes (3H-diazepam, 14C-caffeine, 14C-sucrose, 3H-digoxin, 3H-oleic acid, 3H-2DG, and 14C-(L)-alanine) were purchased from American Radiolabeled Chemicals (Saint Louis, MO, USA). Solvable and Ultima Gold scintillation cocktail were obtained from Perkin Elmer (Waltham, MA, USA). Pierce™ BCA Protein Assay Kit, sequencing grade trypsin, TMTpro™ 16plex Label Reagent Set and other reagents used for proteomics were purchased from Thermo Fisher Scientific (Waltham, MA, USA). A mouse Glut1 ELISA kit was sourced from Wuxi Donglin Sci & Tech Development Co., Ltd. (Wuxi, China). Dulbecco’s Modified Eagle Medium (DMEM), bovine serum albumin (BSA) and phosphate-buffered saline (PBS) were purchased from Sigma-Aldrich (St. Louis, MO, USA).
Pan Y., Kagawa Y., Sun J., Turner B.J., Huang C., Shah A.D., Schittenhelm R.B, & Nicolazzo J.A. (2022). Altered Blood–Brain Barrier Dynamics in the C9orf72 Hexanucleotide Repeat Expansion Mouse Model of Amyotrophic Lateral Sclerosis. Pharmaceutics, 14(12), 2803.
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2

APC Analogs for Cancer Imaging and Therapy

Cited 1 time
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The APC analogs CLR1404, CLR1501 and CLR1502 were provided by Cellectar Biosciences, Inc. (Madison, WI, USA). These APC analogs consist of a cancer-targeting alkylphosphocholine scaffold with an attached cancer imaging, visualization or therapeutic moiety (refs 17 , 18 (link), 25 (link), 28 (link) and Figure 1): iodine isotopes for CLR1404; 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene (i.e. BODIPY, green fluorescence) for CLR1501; and 2-[2-[2-Chloro-3-[2-(1,3-dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)-ethylidene]-1-cyclohexen-1-yl]-ethenyl]-1,3,3-trimethyl-3H-indolium chloride (i.e. IR-775, near infrared fluorescence) for CLR1502. Pharmacological cellular efflux transporter inhibitors were purchased: PSC833 and Ko143 from Tocris Biosciences, and MK571 from Sigma-Aldrich (St. Louis, MO, USA). [14C]-sucrose and [3H]-diazepam were purchased from American Radiolabeled Chemicals (St Louis, MO, USA).
Clark P.A., Alahmad A.J., Qian T., Zhang R.R., Wilson H.K., Weichert J.P., Palecek S.P., Kuo J.S, & Shusta E.V. (2016). Analysis of Cancer-targeting Alkylphosphocholine Analog Permeability Characteristics Using a Human Induced Pluripotent Stem Cell Blood-Brain Barrier Model. Molecular pharmaceutics, 13(9), 3341-3349.
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3

BBB and BCSFB Permeability Measurements

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Measurements of BBB and BCSFB permeability were performed as described recently [1] . In brief, 10 h after P3C (5 mg/kg), LPS (0.3 mg/kg), or saline injection, mice were injected intraperitoneally with [ 14 C]sucrose (0.2 mCi/g; American Radiolabeled Chemicals, St. Louis, MO, USA) and killed 30 min later with an overdose inhalation of isoflurane. Blood was collected from the heart and plasma was separated by centrifugation. CSF and different regions of brain were collected (cortex, striatum/thalamus, cerebellum, and brainstem). All tissues and fluid samples were weighed. Radioactivity in samples was measured by liquid scintillation counting and calculated as cpm/ mg. Tissue radioactivity was corrected for residual blood in tissues [8] . The concentration ratios between CSF/plasma and brain tissue/plasma were calculated as measures of BBB or BCSFB permeability.
Mottahedin A., Smith P.L., Hagberg H., Ek C.J, & Mallard C. (2017). TLR2-mediated leukocyte trafficking to the developing brain. Journal of leukocyte biology, 101(1).
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