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Nucleopore polycarbonate membrane

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Nucleopore polycarbonate membranes are a type of laboratory filtration membrane. They are made of polycarbonate material and contain uniform cylindrical pores of precisely controlled size. These membranes are used for a variety of filtration and separation applications in scientific research and analytical processes.

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Lab products found in correlation

Mini extruder, by Avanti Polar Lipids (2 mentions) Chick serum, by Thermo Fisher Scientific (1 mentions) Streptomycin, by Thermo Fisher Scientific (1 mentions) U lh100hg, by Olympus (1 mentions) Heat inactivated fetal calf serum, by Thermo Fisher Scientific (1 mentions) L glutamine, by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Szx16, by Olympus (1 mentions) Palmitoyl oleoyl phosphatidylcholine, by Avanti Polar Lipids (1 mentions) Palmitoyl oleoyl phosphatidylserine, by Avanti Polar Lipids (1 mentions) Avanti mini extruder, by Avanti Polar Lipids (1 mentions) Sephadex g 25, by GE Healthcare (1 mentions)

Close spelling variants of this product

Polycarbonate nucleopore membrane filter Whatman Nucleopore track-etched polycarbonate membrane Nucleopore polycarbonate track-etch membrane Nucleopore Polycarbonate Nucleopore membrane Polycarbonate membranes Nucleopore

6 protocols using nucleopore polycarbonate membrane

1

Chicken Lung Explant Metabolic Dynamics

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Chicken lung explant culture was performed, as previously described [30 ], to study potential temporal metabolic changes while in controlled settings. Briefly, after dissection in PBS, lungs were placed on top of 8 µm nucleopore polycarbonate membranes (Whatman, USA) and incubated for 48 h in 200 µL of medium 199 (5.5 mM glucose; Sigma, USA) supplemented with 10% (V/V) chick serum (Invitrogen, USA), 5% (V/V) heat-inactivated fetal calf serum (Invitrogen), 1% (V/V) L-glutamine (Invitrogen), 1% (V/V) penicillin 5000 IU/mL plus streptomycin 5000 IU/mL (Invitrogen) and 0.25 mg/mL of ascorbic acid (Sigma). The medium was replaced by fresh supplemented medium at 24 h of culture. Lung explants were photographed at 0 h (D0), 24 h (D1), and 48 h (D2) with a camera (Olympus U-LH100HG) coupled to a stereomicroscope (Olympus SZX16). The medium was collected at D0, D1, and D2 for 1H-NMR spectroscopy analysis. D0 and D2 lung explants were collected for RNA and protein extraction; D2 lung explants were collected for EdU proliferation assay and basal oxygen consumption rate assay.
Fernandes-Silva H., Alves M.G., Araújo-Silva H., Silva A.M., Correia-Pinto J., Oliveira P.F, & Moura R.S. (2021). Lung branching morphogenesis is accompanied by temporal metabolic changes towards a glycolytic preference. Cell & Bioscience, 11, 134.
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2

Preparation of Dansyl-PE Labeled LUVs

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Large Unilamellar Vesicles (LUV) were prepared by drying the required volume of chloroform lipid stocks under a nitrogen stream before overnight drying using high vacuum. Dried lipid films were re-suspended in 50 mM phosphate buffer (pH 8) to a final concentration of 20 mM and vortexed. For FRET measurements, 2% of Dansyl-PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(5-dimethylamino-1-naphthalenesulfonyl) was incorporated into lipid mixture. LUV were formed by extrusion using a Mini-Extruder (Avanti Polar Lipids, Alabaster, AL) through nucleopore polycarbonate membranes of 0.1 μm pore size (Whatman, Philadelphia, PA) [22 , 23 (link)]. LUV stocks were prepared in 50mM phosphate buffer, pH 8, and stored at −4°C. No lipid degradation was detected using thin layer chromatography.
Vasquez-Montes V., Gerhart J., King K.E., Thévenin D, & Ladokhin A.S. (2017). Comparison of lipid-dependent bilayer insertion of pHLIP and its P20G variant. Biochimica et biophysica acta, 1860(2), 534-543.
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3

Lipid Vesicle Preparation by Extrusion

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The appropriate volume of lipid stocks dissolved in chloroform were dried under a nitrogen stream and dried overnight using high vacuum. The dried lipids were re-suspended in 50 mM phosphate buffer, pH 8.0 to a final concentration of 20 mM and large unilamellar vesicles (LUV) were formed by extrusion using a Mini-Extruder (Avanti Polar Lipids, Alabaster, AL). Extrusion was performed using 0.1 μm nucleopore polycarbonate membranes (Whatman, Philadelphia, PA) and the prepared stocks were stored at −4 °C. Lipids used in this study: Palmitoyl-oleoyl-phosphatidylcholine (POPC), palmitoyl-oleoyl-phosphatidylserine (POPS), and 1-palmitoyol-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) were purchased from Avanti Polar Lipids (Alabaster, AL)
Vasquez-Montes V., Gerhart J., Thévenin D, & Ladokhin A.S. (2019). Divalent Cations and Lipid Composition Modulate Membrane Insertion and Cancer-Targeting Action of pHLIP. Journal of molecular biology, 431(24), 5004-5018.
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4

Controlled Vesicle Extrusion for Curosurf

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To control the vesicular size, extrusion of dilute Curosurf® dispersions was performed using an Avanti Mini Extruder (Avanti Polar Lipids, Inc. Alabama, USA). The initial 80 g L -1 stock sample was first diluted with DI-water to 1 g L -1 and extruded 20 times through Whatman Nucleopore polycarbonate membranes (pore size 200 nm) at the temperature of 37 °C. This technique leads to a narrow distribution of vesicles centered around 180 nm, in good agreement with earlier reports [34, 40] .
Szabová J., Mravec F., Mokhtari M., Le Borgne R., Kalina M, & Berret J.F. (2023). N,N,N-Trimethyl chitosan as a permeation enhancer for inhalation drug delivery: Interaction with a model pulmonary surfactant. International journal of biological macromolecules, 239.
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5

Preparation of CL:DOPG Liposomes for S. aureus

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LUVs composed of 100% CL and DOPG:CL = 58:42 were used as model cellular membranes for S. aureus57 (link) and prepared via extrusion. Into a glass vial, CL stock solution was added with or without stock solutions of DOPG; all lipid stock solutions were in chloroform at 20 mg/mL. The resulting lipid mixture was dried under gentle N2 flow, desiccated in vacuum overnight, and rehydrated with CF (40 mM CF) or lucigenin (1 mM lucigenin, 50 mM NaNO3) solutions at 45 °C for 2 h. The resultant solution was subjected to five freeze-thaw cycles and subsequently extruded through a 0.4-μm Nucleopore polycarbonate membrane (Whatman) for 21 times using a mini-extruder (Avanti Polar Lipids). External CF or lucigenin was removed by gel filtration (Sephadex G-25, GE healthcare) using HEPES buffer A (10 mM HEPES, 150 mM NaCl, pH = 7.4) as eluent.
Xie Y, & Yang L. (2016). Calcium and Magnesium Ions Are Membrane-Active against Stationary-Phase Staphylococcus aureus with High Specificity. Scientific Reports, 6, 20628.
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6

Preparation of Ubiquinone-Loaded Liposomes

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For the standard preparation protocol, a total of 5 mg synthetic lipids (stock: 25 mg mL−1 in chloroform) at a mass ratio of 8:1:1 DOPC:DOPE:CL were mixed with 50 nmol ubiquinone Q10 (stock: 6.8 mM in chloroform) in a 25 mL round bottomed flask. For optimization of the co-reconstitution protocol, the amount of Q10 and the lipid composition were varied as described in the figure legends. The chloroform was removed by swirling the flask under a stream of nitrogen, then placing it under vacuum in a desiccator for 2 h. The dried film was rehydrated by the addition of 1 mL reconstitution buffer (10 mM MOPS, pH 7.4, 50 mM KCl) to achieve 5 mg lipid mL−1, and then the flask was filled with nitrogen, sealed, and incubated for 1 h at room temperature. To form the liposomes, the lipid−Q10 mixture was resuspended by vigorous mixing (Vortex Genie 2, Scientific Instruments) followed by 11 extrusions through a 100 nm Nucleopore polycarbonate membrane (Whatman).
Biner O., Fedor J.G., Yin Z, & Hirst J. (2020). Bottom-Up Construction of a Minimal System for Cellular Respiration and Energy Regeneration. ACS synthetic biology, 9(6), 1450-1459.
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