Eos rebel t5 dslr camera

We studied locomotion of aged flies and their progeny in an open field assay, as previously described (2–3 replicates per week for 3 weeks for a total of n = 8 biological replicates)^{35 (link)}. Individual flies were aspirated into the arena (5.80 cm × 0.7 cm Petri dish on a white background and even lighting) and were acclimated for 1 minute before slight mechanical stimulation and placed under a Canon EOS Rebel T5 DSLR camera, which was mounted on a tripod. Videos were recorded for 5 minutes. All videos were converted to mpeg files and the total distance travelled (to determine the general activity level of each fly) was gathered for each fly and analysed with the Noldus EthoVision XT system (Noldus Information Technology, Netherlands).

10 μL mixtures containing 1 μg purified DNA from MG1655 were incubated with 0–1.4 μg Histone H2A or LL-37 for 25 min at 25 °C. Gel loading sample buffer (5×, Bio-Rad, Hercules, CA) was added to a final concentration of 1× and the products were separated by native PAGE on a 5% TBE gel (Bio-Rad, Hercules, CA) at 100 V for 60 min. The gel was stained with 1X SYBR safe (Invitrogen, Carlsbad, CA) in TBE buffer⁷¹ for 30 min before visualization using a EOS Rebel T5 DSLR camera with an f/3.5–5.6 18–55 mm lens (Canon, Huntington, NY) and a DR46B Transilluminator (Clare Chemical, Dolores, CO).

Bright-field color images of the modified pencil lead electrodes were acquired using a Distamax K2 long distance microscope (Centennial, USA) coupled to a Canon EOS Rebel T5 DSLR camera (Melville, USA). Fluorescence images were obtained using a Nikon Ti-U inverted microscope (Melville, USA) equipped with the Chroma 49002 filter set (450–490 nm exc., 500–550 nm emi. and 495 nm dichroic, Bellows Falls, VT, USA) and registered in a monochromatic Qimaging optiMOS camera (Surrey, Canada) through a 4× objective. Fluorescence images were acquired at different focal planes and stitched together using the Extended Depth of Field plugin [41 ] for ImageJ (version 1.52a) [42 (link)]. Scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) mapping were performed in a Zeiss EVO 15 Scanning Electron Microscope (Jena, Germany). Samples were conductive enough that they did not require Au coating to avoid charging.