Log In Sign up
The largest database of trusted experimental protocols

Pfi 1

Manufactured by Bio-Techne
Visit Supplier
Sourced in United Kingdom

PFI-1 is a lab equipment product manufactured by Bio-Techne. It is a small-molecule inhibitor that targets the PROTAC-Formulation Inhibitor 1 (PFI-1) protein.

Automatically generated - may contain errors

Lab products found in correlation

I bet151, by Bio-Techne (3 mentions) Kinase inhibitors, by AG Scientific (2 mentions) I bet, by Bio-Techne (2 mentions) Viktor3, by PerkinElmer (1 mentions)

4 protocols using pfi 1

1

Kinase Inhibitors Purchase and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kinase inhibitors were purchased from A.G. Scientific, Inc. (San Diego, CA), Calbiochem/EMD Chemicals (San Diego, CA), Tocris Bioscience (Ellisville, MO), Archerchem (Mumbai, India), Axon Medchem BV (Groningen, The Netherlands), SYNthesismedchem (Shanghai, China) or Selleckchem (Houston, TX). JQ1 was purchased from BPS Bioscience, Inc. (San Diego, CA) and I-BET 151 and PFI-1 from Tocris. The compound providers confirmed the structures and batch purities (≥ 95%) by using standard analytical methods (HPLC/mass spectroscopy and NMR).
Ciceri P., Müller S., O’Mahony A., Fedorov O., Filippakopoulos P., Hunt J.P., Lasater E.A., Pallares G., Picaud S., Wells C., Martin S., Wodicka L.M., Shah N.P., Treiber D.K, & Knapp S. (2014). Dual kinase-bromodomain inhibitors for rationally designed polypharmacology. Nature chemical biology, 10(4), 305-312.
+ Open protocol
+ Expand
2

Epigenetic Inhibitor Screening Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Media were replaced with serum-free media containing 0.1% DMSO, 0.1 μM/1 μM/10 μM PFI-1 (Tocris Bioscience, Bristol, UK), 0.1 μM/1 μM/10 μM I-BET (Tocris), or 0.01 μM/0.1 μM/1 μM JQ-1 (Cayman Chemical) compounds. Supernatants were collected 24 h later and assayed by ELISA.
Clifford R.L., Patel J.K., John A.E., Tatler A.L., Mazengarb L., Brightling C.E, & Knox A.J. (2015). CXCL8 histone H3 acetylation is dysfunctional in airway smooth muscle in asthma: regulation by BET. American Journal of Physiology - Lung Cellular and Molecular Physiology, 308(9), L962-L972.
+ Open protocol
+ Expand
3

In Vitro Bromodomain Inhibitor Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
For in vitro IC50 studies, 5000 cells per well were seeded in 96-well plates, and maintained overnight to allow cell attachment prior to drug exposure. Bromodomain inhibitors used in this study were JQ1 (ApexBio; Cat. No. A1910), i-BET (i-BET 151; Tocris Bioscience; Cat. No. 4650) and PFI-1 (Tocris Bioscience; Cat. No. 4445/10). Cell viability was assessed by the sulforhodamine B (SRB) assay, 72 h after drug treatment. Briefly, cells were fixed with 10% trichloroacetic acid, washed twice with distilled water and stained with 0.4% SRB in 1% acetic acid for 30 min. SRB was then solubilized in 10mM Tris-HCl pH 10.0 and 540 nm-optical densities were determined using a microplate reader (Perkin Elmer Viktor 3). Data was analyzed with GraphPad Prism 5 software.
Ortiz-Barahona V., Soler M., Davalos V., García-Prieto C.A., Janin M., Setien F., Fernández-Rebollo I., Bech-Serra J.J., De La Torre C., Guil S., Villanueva A., Zhang P.H., Yang L., Guarnacci M., Schumann U., Preiss T., Balaseviciute U., Montal R., Llovet J.M, & Esteller M. (2023). Epigenetic inactivation of the 5-methylcytosine RNA methyltransferase NSUN7 is associated with clinical outcome and therapeutic vulnerability in liver cancer. Molecular Cancer, 22, 83.
+ Open protocol
+ Expand
4

Kinase Inhibitors Purchase and Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols
Kinase inhibitors were purchased from A.G. Scientific, Inc. (San Diego, CA), Calbiochem/EMD Chemicals (San Diego, CA), Tocris Bioscience (Ellisville, MO), Archerchem (Mumbai, India), Axon Medchem BV (Groningen, The Netherlands), SYNthesismedchem (Shanghai, China) or Selleckchem (Houston, TX). JQ1 was purchased from BPS Bioscience, Inc. (San Diego, CA) and I-BET 151 and PFI-1 from Tocris. The compound providers confirmed the structures and batch purities (≥ 95%) by using standard analytical methods (HPLC/mass spectroscopy and NMR).
Ciceri P., Müller S., O’Mahony A., Fedorov O., Filippakopoulos P., Hunt J.P., Lasater E.A., Pallares G., Picaud S., Wells C., Martin S., Wodicka L.M., Shah N.P., Treiber D.K, & Knapp S. (2014). Dual kinase-bromodomain inhibitors for rationally designed polypharmacology. Nature chemical biology, 10(4), 305-312.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!