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2 protocols using mannose

1

TLC Analysis of Mannose Oligomers

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TLC was performed by high performance TLC silicagel plates (Kiselgel 60 F245, Merck). Appropriately diluted samples were applied to the plate (0.5 µl) and eluted twice in ascending mode with a iso-propanol/n-butanol/water mixture (12:4:5). Thymol reagent was used for visualization. A mixture of M1-M6, which contains mannose, mannobiose, mannotriose, mannotetraose, mannopentose and mannohexaose, (Megazyme, Ireland) was used as standards.
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2

Enzymatic Hydrolysis of Konjac Glucomannan

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Konjac glucomannan (KGM, 95% purity) was purchased from Yunnan Genyub Konjac Resource Crop (Kunming, China). Food-grade mannanase with an activity of 10,000 U/g was produced by Bacillus sp. and was obtained from Amano Enzyme (Nagoya, Japan). Mannose (DP1: Mw = 180.16), mannobiose (DP2, Mw = 342.30), mannotriose (DP3, Mw = 504.40), mannotetraose (DP4, Mw = 666.60), mannopentaose (DP5, Mw = 828.70) and mannohexaose (DP6, Mw = 990.90) were purchased from Megazyme (Bray, Ireland). DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) and 2,4,6-tripyridyl-s-triazine (TPTZ) were purchased from Sigma-Aldrich (Singapore).
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