Pmx120 z

The number and size of particles in the exosome solution (dilution factor: 10,000) were determined at 405 nm with a laser using a ZetaView (PMX120-Z, Particle Metrix, Meerbusch, Germany) according to the manufacturer’s protocol. Photos were taken at a rate of 30 pieces/sec for 1 min. The movement of particles was then analyzed using ZetaView software (Version 8.05; Particle Metrix, Inning, Germany).

To characterize the oil droplets within the digestates, the particle concentration, particle size, and size distribution of all particles, including the oil droplets, were determined. Nanoparticle tracking analysis (NTA) was performed with ZetaView PMX 120-Z equipped with a 520 nm laser (Particle Metrix, Inning am Ammersee, Germany). Before the NTA measurements, the black seed oil was digested, as described above. Samples were analyzed after oral, gastric and intestinal in vitro digestion. The instrument settings were optimized to detect particles with a mean size of around 200 nm, and the following parameters were chosen: sensitivity: 60, shutter: 150, frame rate: 15; post-acquisition parameters: min. brightness: 20, min. area: 10, max. area: 10,000, tracelength: 30. All measurements were performed at a flow cell temperature of 22 °C, and the samples were diluted in water to reach about 100–200 particles per frame. For each replicate, 3 measurements were performed, each measuring 11 positions. At least 1000 particles per replicate were measured and included in the analysis for size determination. The data analysis was carried out using ZetaView software, version 8.05.14 SP7.