Several antibodies were used for immunofluorescence, FACS and western blotting (WB). The primary antibodies included a mouse anti-FN monoclonal antibody (ab6328, Abcam; 1:400), a rabbit anti-FN polyclonal antibody (ab2413, Abcam; 1:500), a rabbit anti-Col IV alpha 1 polyclonal antibody (ab189408, Abcam; 1:500), a rabbit anti-YAP1 polyclonal antibody (ab39361, Abcam; 1:400), a rabbit anti-YAP1 (phospho-S127) monoclonal antibody (ab76252, Abcam; 1:3000), a rabbit anti-CD31 polyclonal antibody (ab182981, Abcam; 1:500), a monoclonal anti-Ki-67-APC antibody (130-100-330, Miltenyi; 1:11), a monoclonal REA-APC control (I) antibody (130-104-615, Miltenyi; 1:11), and a mouse anti-GAPDH monoclonal antibody (CW0100M, CWBIO; 1:3000). The secondary antibodies included goat anti-rabbit Alexa Fluor-568 (A11011, Life Technologies; 1:500), goat anti-rabbit Alexa Fluor-488 (A11001, Life Technologies; 1:400), goat anti-mouse Alexa Fluor-488 (A32723, Life Technologies; 1:400), goat anti-mouse Alexa Fluor-568 (A11031, Life Technologies; 1:500), and horseradish peroxidase (HRP)-conjugated antibodies (goat anti-rabbit/mouse; A0208, A0216, Beyotime; 1:3000).
Ab189408
Manufactured by Abcam
Ab189408 is a primary antibody that recognizes the protein target XYZ. It is suitable for use in various immunoassay applications.
Automatically generated - may contain errors
Lab products found in correlation
Ab76252, by Abcam (1 mentions)
Ab39361, by Abcam (1 mentions)
Ab182981, by Abcam (1 mentions)
Ab6328, by Abcam (1 mentions)
A11001, by Thermo Fisher Scientific (1 mentions)
Cw0100m, by CWBIO (1 mentions)
Ab2413, by Abcam (1 mentions)
A0216, by Beyotime (1 mentions)
A32723, by Thermo Fisher Scientific (1 mentions)
A11011, by Thermo Fisher Scientific (1 mentions)
A11031, by Thermo Fisher Scientific (1 mentions)
A0208, by Beyotime (1 mentions)
2 protocols using ab189408
1
Immunofluorescence, FACS, and Western Blotting Antibody Protocol
2
Collagen IV and Synaptopodin Immunofluorescence
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Collagen IV-synaptopodin studies. For the Collagen type IV studies, the Collagen type IV: The Eurodiagnostika Wieslab Alport's Sydrome Kit ALP105RVO was used containing the following antibodies: collagen IV␣1 MAB1 (1:25) mouse; collagen IV␣3 MAB3 (1:100) mouse; collagen IV␣5 MAB5 (1:50) rat; CD31: DAKO, CD31, monoclonal mouse (1:10); synaptopodin: Santa Cruz, synaptopodin (sc21537), goat (1:25); Secondary Fluorescence AK, Invitrogen: AF488 anti-rabbit (1:300); AF488 anti-mouse (1:400); AF488 anti-rat (1:300); AF 546 anti goat (1:300); cell nuclei: DRAQ5 (1:5).
Collagen IV␣1 chain-perlecan-agrin studies. For the Collagen type IV␣1 studies, the antibodies used were the following: Abcam, (ab189408) rabbit polyclonal (1:75); antibodies against the core protein of the basement membrane HSPGs: perlecan: Upstate Biotechnology, (A7L6) rat monoclonal (1:100); agrin: Van den Born, J. (JM72) mouse monoclonal (1:500); Secondary Fluorescence AK: Invitrogen AF488 anti-rabbit (1:250), Dako Diagnostics anti-rat Ig HRP (1:100), Southern Biotech anti-mouse IgG1 HRP (1:100), followed by tyramide-TRITC (1:50), PerkinElmer.
The samples were studied with a conventional fluorescence microscope and a confocal laser-scanning microscope Leica DMRBE or DMRE.
Collagen IV␣1 chain-perlecan-agrin studies. For the Collagen type IV␣1 studies, the antibodies used were the following: Abcam, (ab189408) rabbit polyclonal (1:75); antibodies against the core protein of the basement membrane HSPGs: perlecan: Upstate Biotechnology, (A7L6) rat monoclonal (1:100); agrin: Van den Born, J. (JM72) mouse monoclonal (1:500); Secondary Fluorescence AK: Invitrogen AF488 anti-rabbit (1:250), Dako Diagnostics anti-rat Ig HRP (1:100), Southern Biotech anti-mouse IgG1 HRP (1:100), followed by tyramide-TRITC (1:50), PerkinElmer.
The samples were studied with a conventional fluorescence microscope and a confocal laser-scanning microscope Leica DMRBE or DMRE.
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