To examine the binding of AuNPs and C-CPE-AuNPs on cell surfaces, the 0846 and transfected 0846-FusionRed cell lines were analyzed with SEM. Confluent cells were treated with AuNPs and C-CPE-AuNPs for 3 h in a cell culture incubator to allow complex adhesion to the cells. The cells were exposed to a pulsed laser with 60 mJ/cm² at a scanning speed of 0.5 cm/s. Subsequently, the cells were fixed with 4% formaldehyde, washed with PBS, and stored for further processing. For SEM preparation, the coverslips were dehydrated with a graded series of ethanol completed with an acetone step prior to critical point drying with CO2 as an intermedium (Emitech K850 critical point dryer, Emitech/Quorum Technologies Ltd., Laughton, UK). The coverslips were flat-mounted on SEM-stubs with adhesive carbon tape (Plano, Wetzlar, Germany) and coated with a carbon layer (Leica SCD500, Leica Microsystems, Wetzlar Germany). Specimens were analyzed in a field-emission SEM (Zeiss Merlin VP compact, Carl Zeiss Microscopy, Oberkochen, Germany) equipped with HE-SE and in-lens-Duo detectors operated at 5 kV and images with a size of 1024 × 768 pixels were recorded at different steps of magnification.
Leica scd500
Manufactured by Leica Microsystems
Sourced in Germany
The Leica SCD500 is a sputter coater designed for sample preparation in electron microscopy. It is used to apply a thin conductive coating on non-conductive samples to enhance their surface conductivity, enabling high-quality imaging and analysis in scanning electron microscopes.
Automatically generated - may contain errors
5 protocols using leica scd500
1
Visualizing AuNP and C-CPE-AuNP Binding on Cells
2
Scanning Electron Microscopy of Wing Basal Complex
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The basal complex of the wings was examined with SEM. Prior to the SEM, air-dried wings of the male specimen were sputter coated with a 10 nm gold-palladium layer using a high vacuum sputter coater (Leica SCD 500, Leica Microsystems GmbH, Wetzlar, Germany). For the SEM, a Hitachi TM3000 Tabletop Microscope (Hitachi High-Tech. Corp., Tokyo, Japan) at an accelerating voltage of 15 kV and a magnification of 100X–600X was used.
3
Cryogenic Scanning Electron Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Specimens were air-dried or freeze dried. For freeze drying specimens were rapidly frozen on a liquid nitrogen precooled polished copper block, immediately transferred to a liquid nitrogen precooled exsiccator and freeze dried under vacuum (5 × 10−2 mbar) with subsequent warming to room temperature. Membrane fracturing was performed on rapidly frozen samples under liquid nitrogen with a pair of precooled forceps. Specimens were mounted on SEM stubs with adhesive carbon tape (Plano, Wetzlar, Germany) and coated with a carbon layer of ~15–20 nm (Leica SCD500, Leica Microsystems, Wetzlar Germany). Specimens were viewed in a field-emission SEM (Zeiss Merlin VP compact, Carl Zeiss Microscopy, Oberkochen, Germany) equipped with HE-SE and in-lens-Duo detectors. Images with a size of 1024 × 768 pixels were recorded at different steps of magnification. Measurements of distances were performed using the SmartSEM measurement tools (Carl Zeiss Microscopy).
4
Visualizing AuNPs and C-CPE-AuNPs Binding
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
To examine the binding of AuNPs and C-CPE-AuNPs on cell surfaces, the DT0846 and transfected DT0846-FusionRed cell lines were analyzed with scanning electron microscopy (SEM). Con uent cells were treated with AuNPs and C-CPE-AuNPs for 3 h in cell culture incubator to allow complex adhesion to the cells. The cells were exposed to a pulsed laser with 60 mJ/cm² at a scanning speed of 0.5 cm/s. Subsequently, the cells were xed with 4% formaldehyde, washed with PBS, and stored for further processing. For SEM preparation, the coverslips were dehydrated with a graded series of ethanol completed with an acetone step prior to critical point drying with CO2 as an intermedium (Emitech K850 critical point dryer, Emitech/Quorum Technologies Ltd., Laughton, UK). The coverslips were at mounted on SEM-stubs with adhesive carbon tape (Plano, Wetzlar, Germany) and coated with a carbon layer (Leica SCD500, Leica Microsystems, Wetzlar Germany). Specimens were analyzed in a eld-emission SEM (Zeiss Merlin VP compact, Carl Zeiss Microscopy, Oberkochen, Germany) equipped with HE-SE and inlens-Duo detectors operated at 5 kV and images with a size of 1024 × 768 pixels were recorded at different steps of magni cation.
5
Scanning Electron Microscopy of Haemonchus contortus
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
H. contortus adult females treated with 800 µg/mL TA or thymol for a period of 24 hours were subsequently fixed in a 2.5% glutaraldehyde solution and in a sodium cacodylate buffer 0.1 M (CACO) for 72 h. After three washes in the same buffer, the worms were placed in 2% osmium, and posteriorly in CACO 0.1 M (pH 7.4) buffer fixative for 1 h. Samples were washed two times with CACO and distilled water and were dehydrated in a graded acetone series (30%, 50%, 70%, 90% and 100%). Critical point drying was completed using a CPD 030 (Bal-Tec, Liechtenstein), and samples on metal stubs were coated with a 10 nm layer of gold in a sputter coating machine (Leica SCD 500, Leica Microsystems, Wetzlar, Germany). Parasites were then observed with a scanning electron microscope (FEI QUANTA 200 FEG ESEM, FEI Company, USA) at an accelerating voltage of 20 kV (ANDRE et al., 2016) (link).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!