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Dichlorofluorescin diacetate

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Dichlorofluorescin diacetate is a chemical compound used as a fluorescent probe in biological research. It is used to detect the presence of reactive oxygen species within cells.

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Lab products found in correlation

Ab28455, by Abcam (2 mentions) Ab70359, by Abcam (2 mentions) Ab52771, by Abcam (2 mentions) Phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Sodium pyruvate, by Thermo Fisher Scientific (2 mentions) Phospho c jun ser73, by Cell Signaling Technology (2 mentions) N acetylcysteine nac, by Merck Group (2 mentions) Phospho acc ser79, by Cell Signaling Technology (2 mentions) Z vad fmk, by Santa Cruz Biotechnology (2 mentions) 3 mercaptopicolinic acid, by Santa Cruz Biotechnology (2 mentions) Dimethyl 2 oxoglutarate, by Tokyo Chemical Industry (2 mentions) Dimethyl fumarate, by Tokyo Chemical Industry (2 mentions) Dimethyl succinate, by Tokyo Chemical Industry (2 mentions) Dimethyl malate, by Tokyo Chemical Industry (2 mentions) Necrostatin 1, by Santa Cruz Biotechnology (2 mentions) Ampkα, by Cell Signaling Technology (2 mentions) L glutamine, by Thermo Fisher Scientific (2 mentions) Flow cytometry, by BD (1 mentions)

Close spelling variants of this product

2′,7′-dichlorofluorescin diacetate (DCFH-DA) (45 citations) 2,7-dichlorofluorescin diacetate (7 citations) 2',7'-dichlorofluorescin diacetate (DCF-DA) (3 citations)

3 protocols using dichlorofluorescin diacetate

1

Metabolic Regulation Pathway Investigation

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3-mercaptopicolinic acid, Z-VAD-FMK and Necrostatin-1 were purchased from Santa Cruz Biotechnology. Dimethyl-2-oxoglutarate, dimethyl-fumarate, dimethyl-succinate and dimethyl-malate were purchased from Tokyo Chemical Industry, Sodium pyruvate and L-glutamine were purchased from Thermo Fisher Scientific, N-acetylcysteine (NAC) was purchased from Sigma-Aldrich, and dichlorofluorescin diacetate from Beyotime.
Antibodies against PCK1 (ab28455, Abcam), PCK2 (ab70359, Abcam) and YAP1 (ab52771, Abcam), phospho-AMPKα (Thr172) (Cell Signaling Technology, 2535), AMPKα (Cell Signaling, 2532), phospho-ACC (Ser79) (Cell Signaling, 11818) and phospho-c-Jun (Ser73) (Cell Signaling, 3270) were purchased commercially.
pBABE-Flag-PCK1 and pQCXIH-Flag-PCK2 were cloned from cDNA provided by Dr. Jiahuai Han (Xiamen University, Xiamen, China). The PB[CMV-myc-YAP-5SA]DS, PB[Act-RFP]DS and Act-PB Transposase plasmids were generous gifts from Dr. Bin Zhao (Zhejiang University, Hangzhou, China). The PB[CMV-flag-PCK1]DS donor plasmids were constructed by excising Act-RFP from the PB[Act-RFP]DS plasmid and ligating the corresponding fragments excised from pQCXIH vectors.
Liu M.X., Jin L., Sun S.J., Liu P., Feng X., Cheng Z.L., Liu W.R., Guan K.L., Shi Y.H., Yuan H.X, & Xiong Y. (2018). Metabolic reprogramming by PCK1 promotes TCA cataplerosis, oxidative stress and apoptosis in liver cancer cells and suppresses hepatocellular carcinoma. Oncogene, 37(12), 1637-1653.
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2

Metabolic Regulation Pathway Investigation

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3-mercaptopicolinic acid, Z-VAD-FMK and Necrostatin-1 were purchased from Santa Cruz Biotechnology. Dimethyl-2-oxoglutarate, dimethyl-fumarate, dimethyl-succinate and dimethyl-malate were purchased from Tokyo Chemical Industry, Sodium pyruvate and L-glutamine were purchased from Thermo Fisher Scientific, N-acetylcysteine (NAC) was purchased from Sigma-Aldrich, and dichlorofluorescin diacetate from Beyotime.
Antibodies against PCK1 (ab28455, Abcam), PCK2 (ab70359, Abcam) and YAP1 (ab52771, Abcam), phospho-AMPKα (Thr172) (Cell Signaling Technology, 2535), AMPKα (Cell Signaling, 2532), phospho-ACC (Ser79) (Cell Signaling, 11818) and phospho-c-Jun (Ser73) (Cell Signaling, 3270) were purchased commercially.
pBABE-Flag-PCK1 and pQCXIH-Flag-PCK2 were cloned from cDNA provided by Dr. Jiahuai Han (Xiamen University, Xiamen, China). The PB[CMV-myc-YAP-5SA]DS, PB[Act-RFP]DS and Act-PB Transposase plasmids were generous gifts from Dr. Bin Zhao (Zhejiang University, Hangzhou, China). The PB[CMV-flag-PCK1]DS donor plasmids were constructed by excising Act-RFP from the PB[Act-RFP]DS plasmid and ligating the corresponding fragments excised from pQCXIH vectors.
Liu M.X., Jin L., Sun S.J., Liu P., Feng X., Cheng Z.L., Liu W.R., Guan K.L., Shi Y.H., Yuan H.X, & Xiong Y. (2018). Metabolic reprogramming by PCK1 promotes TCA cataplerosis, oxidative stress and apoptosis in liver cancer cells and suppresses hepatocellular carcinoma. Oncogene, 37(12), 1637-1653.
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3

Quantifying Neutrophil Oxidative Stress

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The PMN were harvested when incubated with 3 mM BHB (with or without 10 μM 740Y-P) for 4 h. After treatment, PMN were incubated with dichlorofluorescin diacetate (Beyotime Biotechnology Inc.) for 30 min at 37°C. The PMN were collected by centrifugation and washed 3 times with cold PBS, followed by resuspension by 200 μL of PBS. For determination of ROS content, the fluorescence intensity of PMN was measured by flow cytometry (Becton Dickinson).
Liu S., Li X., Zhou X., Loor J.J., Jiang Q., Feng X., Yang Y., Lei L., Du X., Li X., Zhe W., Song Y, & Liu G. (2022). β-Hydroxybutyrate impairs the release of bovine neutrophil extracellular traps through inhibiting phosphoinositide 3-kinase-mediated nicotinamide adenine dinucleotide phosphate oxidase reactive oxygen species production. Journal of dairy science, 105(4).
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