Dba 1 j mice

Six-week-old male DBA/1 J mice were purchased from Orient Bio Inc. (Seongnam, Korea). CIA was induced as described previously [22 (link)]. To induce CIA in mice, CII was dissolved overnight in 0.1 N acetic acid (4 mg/mL) with gentle rotation at 4 °C. DBA/1 J mice were injected intradermally at the base of the tail with 100 μg of CII emulsified in Freund’s adjuvant (Chondrex). Two weeks later, 100 μg of type II collagen dissolved and emulsified 1:1 with incomplete Freund’s adjuvant (Difco) was administered to the hind leg of mice as a booster injection. On day 24 after the first immunization, mice were injected subcutaneously with 2.5 mg/kg MTX or MTX-NPs twice weekly. Animals were maintained under specific pathogen-free conditions at the Institute of Medical Science of the Catholic University of Korea and were fed standard mouse chow and water. All experimental procedures were examined and approved by the Animal Research Ethics Committee of the Catholic University of Korea; the procedure conformed to all National Institutes of Health of the United States guidelines (Permit number: 2020-0067-01).

CIA was generated in male DBA/1J mice (Orient Bio, Korea). Mice were maintained in a specific-pathogen-free environment. DBA/1J mice received intradermal injection of 100 μg of chicken type II collagen (CII; Chondrex Inc., Redmond, WA, USA) mixed with complete or incomplete Freund's adjuvant (Chondrex Inc.). To assess the influence of SAHA on the severity of symptoms in the CIA model, mice were treated with 50 mg/kg SAHA or vehicle alone via daily oral feeding after booster immunization. Mice were sacrificed at 8 weeks after induction of CIA.
Mice were considered to have arthritis when significant changes in redness and/or swelling were noted in the digits or other parts of the paws. Knee joint inflammation was scored visually after dissection on a scale from 0 to 4 (0, noninflamed; 1, minimal; 2, mild; 3, moderate; and 4, severe inflammation).
All experimental procedures were approved by the Animal Research Ethics Committee at the Catholic University of Korea (approval number 2018-0115-02).

Nine weeks old female wild-type DBA1/j mice were purchased from Central Lab Animal Inc. (Seoul, Korea). 100 µL of emulsified solution (1:1, v/v) of collagen type II (4 mg/mL) (Chondrex) and CFA (1 mg/mL) (Chondrex) mixture was intradermally (i.d.) injected into DBA/1j mice. The incidence of arthritis was complete after 5 weeks, and CDr17 (1 mM) was injected into CIA mice by i.v. injection. The in vivo imaging was performed by an AMI HTX (Spectral Instruments Imaging, Tucson, AZ, USA) with λ_ex = 640 nm and λ_em = 690 nm. The data were analyzed by Aura software (Spectral Instruments Imaging). The separate three experiments were proceeded with similar results. No data were excluded from the analyses.