Immunoblotting assays were carried out as previously described9 with the following primary Abs: DAB2IP (23582‐1‐AP, Proteintech), c‐Myc (ab32072, Abcam), c‐Myc (67447‐1‐Ig, Proteintech), p‐c‐MycS62 (ab185656, Abcam), p‐c‐MycT58 (ab185655, Abcam), glycogen synthase kinase 3β (GSK3β; 10044‐T32, SinoBiological), p‐GSK3βS9 (#5558, CST), Bcl‐2 (12789‐1‐AP, Proteintech), Bax (#5023, CST), B56α (ab89621, Abcam), Ubiquitin (10201‐2‐AP, Proteintech), Ubiquitin (linkage‐specific K48) (ab140601, Abcam), Nanog (#8822S, CST), CD44 (60224‐1‐Ig, Proteintech), CD133 (18470‐1‐AP, Proteintech), and GAPDH (60004‐1‐Ig, Proteintech). Secondary Abs conjugated to HRP (Servicebio) were used, followed by chemiluminescence.
Ab185655
Manufactured by Abcam
Sourced in United Kingdom
Ab185655 is a laboratory equipment product. It serves a core function in scientific research and analysis. Further details on its intended use or application are not available.
Automatically generated - may contain errors
Lab products found in correlation
Ab32072, by Abcam (2 mentions)
Ab89621, by Abcam (1 mentions)
Ubiquitin, by Proteintech (1 mentions)
Ab185656, by Abcam (1 mentions)
Ab140601, by Abcam (1 mentions)
Gsk3β, by Sino Biological (1 mentions)
Dab2ip, by Proteintech (1 mentions)
Gapdh, by Proteintech (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
β actin ab8227, by Abcam (1 mentions)
Ab191606, by Abcam (1 mentions)
Ab182408, by Abcam (1 mentions)
Ab133666, by Abcam (1 mentions)
Ab103314, by Abcam (1 mentions)
Ab32500, by Abcam (1 mentions)
Ab267373, by Abcam (1 mentions)
Ab175607, by Abcam (1 mentions)
Ab138005, by Abcam (1 mentions)
2 protocols using ab185655
1
Comprehensive Immunoblotting Assay Protocol
2
Protein Extraction and Immunoblotting Analysis
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Proteins were extracted from cultured cells, which was followed by immunoblotting with the primary antibodies. Protein concentrations were determined by Bradford assay. Proteins were separated by SDS-PAGE, transferred onto PVDF membranes (Millipore Corporation, Burlington, MA, USA), and probed with the indicated antibodies. Antibodies that recognise ASS1 (ab175607), PYCR1 (ab103314), IGF1R (ab182408), c-MYC (ab32072), c-MYCT58 (ab185655), JAK1 (ab133666), JAK1-P (ab138005), STAT3 (ab32500), STAT3-P (ab267373), PI3K (ab191606), PI3K-P (ab182651), AKT (ab179463), AKT-P (ab192623), mTORC (ab134903), and β-actin (ab8227) were purchased from Abcam (Cambridge, UK). Immunoblotting assays were performed as previously reported [18 (link)].
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