Intracellular levels of lipid peroxidation/ROS were assessed using BODIPY581/591 C11/CellRox green dye (ThermoFisher Scientific). BODIPY581/591 C11 or CellRox green dye (5 μM; diluted in DMEM with 10% fetal bovine serum) was added to U251 and LN229 cells pretreated with 5 μM DSF or DMSO. Fluorescence was observed under confocal microscopy (Leica SP5 Confocal Microscope, Leica).
Bodipy581 591 c11 cellrox green dye
Manufactured by Thermo Fisher Scientific
BODIPY581/591 C11/CellRox green dye is a fluorescent probe used for the detection of lipid peroxidation and oxidative stress in cells. It is a combination of two dyes: BODIPY581/591 C11, which is sensitive to lipid peroxidation, and CellROX Green, which detects general oxidative stress. The dye emits a green fluorescent signal upon oxidation, allowing for the visualization and quantification of these cellular processes.
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Lab products found in correlation
2 protocols using bodipy581 591 c11 cellrox green dye
1
Assessing Intracellular Lipid Peroxidation
2
Lipid Peroxidation Assay in Glioma Cells
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LN229 and U118 cells were seeded into 6-well, flat-bottomed plates and incubated overnight. After treatment with 1 μM FIN56 or DMSO for 24 h, intracellular levels of lipid peroxidation/ROS were assessed using BODIPY581/591 C11/CellRox green dye (ThermoFisher Scientific). BODIPY581/591 C11 or CellRox green dye (5 μM; diluted in DMEM with 10% fetal bovine serum) was added to LN229 and U118 cells. Then images were obtained under confocal microscopy (Leica SP5 Confocal Microscope, Leica).
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