Alexa fluor 647 a21236
Alexa Fluor 647 (A21236) is a fluorescent dye used in various biomedical applications. It has an excitation maximum of 650 nm and an emission maximum of 665 nm, making it suitable for detection and analysis in the far-red region of the visible spectrum.
Lab products found in correlation
2 protocols using alexa fluor 647 a21236
Immunofluorescence Staining of Cellular Proteins
Immunohistochemistry Protocol for Fluorescence Imaging
The primary antibodies used were as follows: chicken anti-GFP (1:1,000) (ab13970; Abcam), rabbit anti-HA (1:1,000) (3724S; Cell Signaling Technologies), rabbit anti-DsRed (1:200) (632496; Takara), and mouse anti-chaoptin (1:20) (24B10; Developmental Studies Hybridoma Bank). Rat anti-dFezf (1:50) was a gift from Dr. Claude Desplan (New York University, New York, NY).
The secondary antibodies used were as follows: Goat anti-chicken immune globulin (Ig) G (H + L) Alexa Fluor 488 (A-11039), goat anti-rabbit IgG (H + L) Alexa Fluor 568 (A-11011), goat anti-mouse IgG (H + L) Alexa Fluor 647 (A-21236), and goat anti-rat IgG (H + L) Alexa Fluor 568 (A-11077) were purchased from Thermo Fischer Scientific and used at a dilution of 1:500.
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