and E.C. 1.4.3.11, manufactured from YAMASA Corporation, Tokyo, Japan)
and
manufactured from Oriental Yeast Co., Ltd., Tokyo, Japan), nicotinamide
adenine dinucleotide phosphate reduced form (NADPH),
acid sodium salt monohydrate (L-glu), and hydrogen peroxide solution
(30%) were purchased from Wako Pure Chemical Industries (Osaka, Japan).
Ethylene glycol diglycidyl ether (EGDE) and α-ketoglutaric acid
(α-KA) were obtained from Tokyo Chemical Industry (Tokyo, Japan).
Chitosan was acquired from Junsei Chemical (Tokyo, Japan). Aqueous
ammonia solution (14.8 M) was procured from Kanto Chemical (Tokyo,
Japan). The Prussian blue (PB)-carbon electrode (electrode diameter:
6 mm) manufactured from Gwent Electronic Materials (London, UK) was
provided by DIC Corporation (Tokyo, Japan). Other reagents were obtained
from Wako Pure Chemical Industries (Osaka, Japan). Type 1 ultrapure
water (MilliQ water, Merck Ltd., Tokyo, Japan) was used in all experiments.