Seven days after tumor implantation, orthotopic brain tumor-bearing animals (n=5 mice/experimental group) received a single dose (5×1011 TU i.v. per mouse) of RGD4C-AAVP-HSVtk. Either GCV (80 mg/kg/d i.p.) or saline control was administered daily from days 18 to 23 after tumor implantation. One day after the last treatment with GCV, animals were killed and whole brains removed for histopathological analysis. To evaluate HSVtk gene expression, PET scan imaging was performed 2 hours after i.v. administration of the radiolabeled nucleoside analog [18F]-FEAU.(22 (link)) PET scan imaging was performed with an Inveon micro-PET/CT scanner (Siemens Preclinical Solution). Glioma-bearing mice were anesthetized (with isoflurane 2% in 98% oxygen), and their temperature was kept at 38°C with a heat lamp. Fully three-dimensional list mode data were collected by using an energy window of 350–750 kiloelectron volts and a time window of 6 nanoseconds. Images were reconstructed by a 2D ordered subsets expectation maximization algorithm. PET image analyses were performed with vendor software ASIPro 5.2.4.0 (Siemens Preclinical Solutions).
Asipro 5
Manufactured by Siemens
Sourced in Germany
ASIPro 5.2.4.0 is a laboratory equipment product from Siemens. It is a software package designed for data acquisition and analysis. The core function of ASIPro 5.2.4.0 is to provide a platform for collecting, managing, and processing data from various laboratory instruments and sensors.
Automatically generated - may contain errors
8 protocols using asipro 5
1
Multimodal Imaging of HSVtk Expression
2
Glioma-targeting Viral Therapy and Imaging
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Seven days after tumor implantation, orthotopic brain tumor-bearing animals (n = 5 mice/experimental group) received a single dose (5 × 1011 TU i.v. per mouse) of RGD4C-AAVP-HSVtk. Either GCV (80 mg/kg/d i.p.) or saline control was administered daily from days 18 to 23 after tumor implantation. One day after the last treatment with GCV, animals were killed and whole brains removed for histopathological analysis. To evaluate HSVtk gene expression, PET imaging was performed 2 h after i.v. administration of the radiolabeled nucleoside analog [18F]-FEAU [22 (link)]. PET imaging was performed with an Inveon micro-PET/CT scanner (Siemens Preclinical Solution). Glioma-bearing mice were anesthetized (with isoflurane 2% in 98% oxygen), and their temperature was kept at 38 °C with a heat lamp. Fully 3D list mode data were collected by using an energy window of 350–750 keV and a time window of 6 ns. Images were reconstructed by a 2D ordered subsets expectation maximization algorithm. PET image analyses were performed with vendor software ASIPro 5.2.4.0 (Siemens Preclinical Solutions).
3
Small-Animal PET Imaging of [18F]Fluoroethyl Bufalin
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Small-animal PET scans for the [18F]fluoroethyl bufalin were performed in both SMMC-7721 and HepG2 tumor model (three mice per group). The animals were anesthetized under 1.5% isoflurane. Then the mice were placed prostrate and fixed. After that the mice were injected with 7.4 MBq [18F]fluoroethyl bufalin (0.2 mL) through the tail vein and immediately scanned dynamically for 360 min. The scans were rebuilt by a two-dimensional ordered-subset expectation maximization (2D OSEM) process without rectification for decay or dispersing. For the images, regions of interest (ROIs) were drafted over the major tissues and tumor by the vendor-supplied software (ASI Pro 5.2.4.0 Siemens Healthineers, Erlangen, Germany) on attenuated corrected entire body coronal images. The radioactivity attention in the major organs and tissues was got from average pixel values within the ROI level. After that, the data were translated to megabecquerel per milliliter per minute by the standardization factors measured in the Inveon PET equipment. The data were split by the radioactivity to get (presuming an organizational density of 1 g/mL) a %ID/g ROI image.
4
Glucose Uptake Quantification in Normoxia and Hypoxia
5
In Vivo Tumor Imaging with 18F-FDG PET/CT
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The xenograft-bearing mice were fasted overnight and anesthetized with inhaled isoflurane. 18F-FDG of about 200 μCi per mouse was injected into the tail vein. After 60 min of nonspecific clearance, the mouse was scanned in microPET/CT Inveon scanner (Siemens, Knoxville, TN, USA) and images were then reconstructed using a two-dimensional ordered subsets expectation maximization algorithm. PET and CT image fusion and image analysis were performed using software ASIPro 5.2.4.0 (Siemens).
6
PET Imaging of Tumor-Targeting Radiotracers
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PET scans
were obtained using
an Inveon small animal PET scanner (Siemens Medical Solutions). Under
isoflurane anesthesia, 5 min of static PET scanning was performed
at 15, 30, and 60 min after the PC-3 tumor-bearing mice were each
intravenously injected with 3.7 MBq of 68Ga–NOTA–Aca–BBN7–14 (28.2 MBq/nmol) (n = 4) or 68Ga–NOTA–PEG3–RM26 (32.6 MBq/nmol)
(n = 3) in a volume of 100 μL of PBS.
The PET images were reconstructed using 3-dimensional ordered-subsets
expectation maximum (3D OSEM) followed by maximum a posteriori (MAP)
algorithm with a smoothing parameter (OSEM-3D-MAP) of 0.1. For each
scan, regions of interest (ROIs) were drawn over the tumor on whole-body
decay-corrected coronal images using vendor software (ASI Pro 5.2.4.0;
Siemens Medical Solutions). The radioactivity accumulation within
the tumor was calculated from mean pixel values of the multiple ROI
volumes. These values were converted to MBq/mL and then further divided
by the administered activity to obtain an image-ROI-derived %ID/g
value (assuming a tissue density of 1 g/mL). No correction was applied
in this study.
were obtained using
an Inveon small animal PET scanner (Siemens Medical Solutions). Under
isoflurane anesthesia, 5 min of static PET scanning was performed
at 15, 30, and 60 min after the PC-3 tumor-bearing mice were each
intravenously injected with 3.7 MBq of 68Ga–NOTA–Aca–BBN7–14 (28.2 MBq/nmol) (n = 4) or 68Ga–NOTA–PEG3–RM26 (32.6 MBq/nmol)
(n = 3) in a volume of 100 μL of PBS.
The PET images were reconstructed using 3-dimensional ordered-subsets
expectation maximum (3D OSEM) followed by maximum a posteriori (MAP)
algorithm with a smoothing parameter (OSEM-3D-MAP) of 0.1. For each
scan, regions of interest (ROIs) were drawn over the tumor on whole-body
decay-corrected coronal images using vendor software (ASI Pro 5.2.4.0;
Siemens Medical Solutions). The radioactivity accumulation within
the tumor was calculated from mean pixel values of the multiple ROI
volumes. These values were converted to MBq/mL and then further divided
by the administered activity to obtain an image-ROI-derived %ID/g
value (assuming a tissue density of 1 g/mL). No correction was applied
in this study.
7
PET Imaging of Tracer Biodistribution
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PET scans were obtained and image analyses were performed using an Inveon small-animal PET scanner (Siemens Medical Solutions). About 3.7 MBq of 18F-AA was administered via tail-vein injection under isoflurane anesthesia. For static acquisition, 10-min static PET scans were acquired at 30, 60, and 120 min after injection. For dynamic acquisition, 60-min dynamic PET scans were acquired, followed by late–time point scans at 2 hours after tracer injection. With the acute inflammation model, 10-min static PET images were acquired at 1 and 2 hours after injection. The images were reconstructed using a three-dimensional (3D) ordered subset expectation maximum algorithm, and no correction was applied for attenuation or scatter. For each scan, ROIs were drawn using vendor software (ASI Pro 5.2.4.0, Siemens Medical Solutions) on decay-corrected whole-body coronal images. The radioactivity concentrations (accumulation) in the tumor, heart, muscle, liver, brain, and kidneys were obtained from mean pixel values within the multiple ROI volumes and then converted to megabecquerel per milliliter. These values were then divided by the administered activity to obtain (assuming a tissue density of 1 g/ml) an image ROI–derived percentage injected dose per gram (% ID/g).
8
Quantifying Radiotracer Uptake in PET Imaging
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The images were reconstructed using a 3-dimensional ordered subset expectation maximum (OSEM) algorithm, and no correction was applied for attenuation or scatter. For each scan, regions of interest (ROIs) were drawn using vendor software (ASI Pro 5.2.4.0; Siemens Medical Solutions) on decay-corrected whole-body coronal images. The radioactivity concentrations (accumulation) within the tumor and heart were obtained from mean pixel values within the multiple ROI volumes and then converted to megabecquerel per milliliter. These values were then divided by the administered activity to obtain (assuming a tissue density of 1 g/ml) an image-ROI-derived percentage injected dose per gram (%ID/g).
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