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2 protocols using 6 hydroxy 2 5 7 8 tetramethylchroman 2 carboxylic acid trolox 2 4 6 tri 2 pyridyl s triazine tptz

1

Phytochemical Analysis and Antioxidant Evaluation

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The organic solvents acetone, ethanol and methanol were purchased from Merck (Darmstadt, Germany). Folin-Ciocalteu's reagent and other chemicals including anhydrous sodium carbonate (Na2CO3), sodium nitrite (NaNO2), hydrochloric acid (HCl), potassium persulfate (K2S2O8), copper (II) chloride (CuCl2), ferric chloride (FeCl3), Aluminium chloride hexahydrate (AlCl3·6H2O), ammonium acetate, 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), (±)-6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), 2,4,6-Tri(2-pyridyl)-s-triazine (TPTZ), neocuproine, gallic acid, and catechin were purchased from Sigma-Aldrich Pty Ltd. (Castle Hill, Sydney, Australia). Vanillin and sodium hydroxide (NaOH) were obtained from Merck (Darmstadt, Germany). All the solvents and chemicals used in this study were analytical grade.
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2

Comprehensive Phytochemical Analysis Protocol

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Acetonitrile, formic acid, methanol, all-trans-β-carotene, all-trans-lutein, all-trans-zeaxanthin, violaxanthin, chlorophyll a, chlorophyll b, chlorophyllide b, pheophytin a, pheophytin b, betulinic, oleanolic and ursolic acid, ABTS 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox), 2,4,6-tri(2-pyridyl)-s-triazine (TPTZ), methanol acetic acid, and phloroglucinol were purchased from Sigma-Aldrich (Steinheim, Germany). (-)-Epicatechin, (+)-catechin, procyanidin B2,, caffeic acid, p-coumaric acid, 3-O-caffeoylquinic, 5-O-caffeoylquinic, ferulic acid, galloyl glucose, caftaric acid, luteolin 7-O-galactoside, apigenin 7-O-glucoside, kaempferol-3-O-galactoside, quercetin-3-Orutinoside, quercetin-3-O-galactoside, quercetin-3-O-glucoside, quercetin-3-O-arabinoside, quercetin-3-O-xyloside, cis-piceid, trans-piceid, cis-resveratrol were purchased from Extrasynthese (Lyon, France). Acetonitrile for ultra-phase liquid chromatography (UPLC; Gradient grade) and ascorbic acid were purchased from Merck (Darmstadt, Germany).
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