The normal human FLSs and rheumatoid arthritis (RA) FLSs were purchased from Cell Applications (San Diego, CA, U.S.A.). The cells were cultured in DMEM containing 10% FBS and stored in a humidified incubator with 5% CO2 at 37°C.
Normal human flss
Manufactured by Cell Applications
Sourced in United States
Normal human FLSs are primary cells derived from the synovial membrane of healthy individuals. These cells are important components of the joint and play a crucial role in maintaining joint homeostasis. The core function of normal human FLSs is to provide structural support and regulate the synovial environment within the joint.
Automatically generated - may contain errors
3 protocols using normal human flss
1
Culturing Normal and RA Fibroblast-Like Synoviocytes
2
Cell Culture and Transfection Methods
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Normal human FLSs and RA-FLSs were purchased from Cell Applications (San Diego, CA, U.S.A.) and cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS, 100 U/ml penicillin and 100 mg/ml streptomycin at 37°C with 5% CO2. Transfection of DNA constructs and siRNA was performed using Lipofactamine 2000 (Invitrogen, Carlsbad, CA, U.S.A.) following the manufacturer’s instructions.
3
Transfection of miR-506 in RA-FLSs
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Normal human FLSs and RA-FLSs were brought from Cell Applications (San Diego, CA, U.S.A.). For cell-culture, DMEM (Invitrogen, Carlsbad, CA, U.S.A.) was used and augmented with FBS (10%; HyClone, UT, U.S.A.), 100 units/ml penicillin or 100 μg/ml streptomycin. The cells were grown at 37°C in an atmosphere with appropriate humidity, and 5% CO2. MiR-506 mimic and oligonucleotide negative control (miR-NC) were brought from the GenePharm (Shanghai, China) and transfected into the RA-FLSs cells by using Lipofectamine 2000 (Invitrogen, CA, U.S.A.) as per instructions of the manufacturer.
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