Anti-TLR4 was purchased from Santa Cruz (Dallas, TX, United States), anti-NF-κB and β-actin were purchased from Cell Signaling Technologies (Darmstadt, Germany), anti-MyD88 and anti-collagen Ⅰ were obtained from Abcam (Cambridge, MA, United States). HRP-conjugated anti-mouse IgG and rabbit IgG were purchased from Cell Signaling Technologies. The enhanced chemiluminescence reagent and Image Lab System were obtained from Bio-Rad (Hercules, CA, United States), and the BCA protein detection kit was procured from Thermo Fisher Scientific (Waltham, MA, United States). IL-1β and IL-6 assay kits were purchased from Abcam, TNF-α, IFN-γ assay kits were obtained from Raybiotech (Georgia, United States), and the LPS assay kit was obtained from Aviva Systems Biology (CA, United States). The hematoxylin and eosin (HE) staining kit was obtained from BOSTER (Wuhan, China), and the Masson staining kit was purchased from Nanjing Jiancheng Bioengineering Institute (Jiangsu, China).
Emodin was purchased from Shanghai Winherb Medical Technology Company (Shanghai, China). PLA was purchased from Jinan Daigang Biomaterial Company, Shandong, China, and mPEG was purchased from Xian Ruixi Biomaterial Company, Shanxi, China.
Emodin was purchased from Shanghai Winherb Medical Technology Company (Shanghai, China). PLA was purchased from Jinan Daigang Biomaterial Company, Shandong, China, and mPEG was purchased from Xian Ruixi Biomaterial Company, Shanxi, China.