Tunel assay kit ab66110

Human proximal tubular epithelial cells (HK-2 cells) were cryopreserved at the Institute of Kidney Disease, Central South University. SS31 was synthesised and provided by Chinapeptide Co. Ltd. (Shanghai, China). Streptozocin (STZ) was obtained from Sigma-Aldrich (USA). The selective Drp1 inhibitor Mdivi1 (ab144589) was obtained from Abcam (UK). Anti-fibronectin (FN) antibody (sc-52331), anti-Bcl-2 antibody (sc-56015), anti-IL-1β antibody (sc-52012), and anti-Bax antibody (sc-20067) were obtained from Santa Cruz Biotechnology (Santa Cruz, CA). Anti-Drp1 rabbit monoclonal antibody (ab184247), anti-Mfn1 mice monoclonal antibody (ab57602), and Caspase1 antibody (ab138483) were purchased from Abcam (UK). The TUNEL assay kit (ab66110) and anti-β-actin antibody (ab8226) were purchased from Abcam (UK). Secondary antibodies in this study were purchased from KangChen Bio-tech (Shanghai, China). Other materials, including bovine serum albumin and low-glucose DMEM medium, were purchased from Gibco (USA).

H9C2 rat cardiomyoblast cells were purchased from ATCC (Manassas, VA, USA). H9C2 cells were cultured in DMEM supplemented with 10% fetal bovine serum (FBS), 100 mg/mL streptomycin and 100 U/mL penicillin in a humidified atmosphere supplemented with 5% CO₂ at 37°C. FBS and trypsin-EDTA were purchased from Thermo Fisher Scientific. LDH assay kit (ab102526) and TUNEL assay kit (ab66110) were purchased from Abcam. Antibodies against MAPK1 (TA500485), β-actin (AM4302) and HRP-labelled secondary antibody were purchased from Thermo Fisher Scientific.