The paraffin-embedded tissue was cut into 8 μm-thick sections. After dewaxing and hydrating, immunostaining was conducted. Sections were pretreated with 0.1% Triton X-100 in PBS with 1% bovine serum albumin for 1 h, 1% t20 for 20 min, and then PBS for 20 min. Briefly, the sections were incubated for 30 min in the dark. The excess dye was washed away with PBS. Sections were incubated with isotype antibodies to exclude false positive staining. At least three parallel sections from different implantation sites were observed by fluorescence microscopy (Zeiss stereoscopic finding, V20, Germany). Five fields were randomly selected for immunofluorescence determination. Macrophages were magnified 400-fold to exclude false positive staining. Semiquantitative analysis was then performed at a magnification of 40-fold. CaseViewer 2.1 and Image Pro Plus 7.0 were used to measure the fluorescence intensity of five random spots. Hoechst (ab138903, Abcam) staining analysis was performed at the same time as CD68 (ab222914, Abcam) and inducible nitric oxide synthase (iNOS, ab209027, Abcam) analysis.
Ab222914
Manufactured by Abcam
Sourced in United Kingdom
Ab222914 is a laboratory equipment product offered by Abcam. It is a device designed for a specific laboratory function, but a detailed description cannot be provided while maintaining an unbiased and factual approach. More information from the manufacturer would be required to give a accurate and concise description of the product's core function.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab222914
1
Immunostaining of Macrophages in Paraffin-Embedded Tissue
2
Quantifying Macrophage Phenotypes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The expression of CD68 and CD206 were detected. Brie y, cells were collected and washed 3 times with PBS. Cell suspension was then blocked with bovine serum albumin (BSA) for 30 minutes. Incubated cells with Mouse Anti-Mouse CD68 (1:100, ab222914, Abcam, UK) and Rabbit Anti-Mouse CD206 (1:200, ab64693, Abcam, UK) for 1 h. Washed for 3 times.
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