According to the manufacturer’s instructions, ELISA kits (88-7324, 88-7064, 88-7013, Thermo Fisher Scientific, Waltham, MA, USA; ab100732, Abcam; ab253227, Abcam) were used to evaluate the levels of inflammatory factors (interleukin IL-6, IL-1β, and TNF-α), MMP-9 and proMMP-9 in serum. Briefly, the samples were incubated with appropriate monoclonal antibodies and biotinylated anti-mice antibodies, sequentially, followed by horseradish oxidase. The measured optical density (OD) values were converted into concentration using standard curves.
Ab100732
Manufactured by Abcam
Sourced in United States, China
Ab100732 is a primary antibody that targets the protein [Insert target protein name]. It is suitable for use in various immunoassay applications, including Western blotting, immunohistochemistry, and ELISA. The antibody is produced in [Insert host species] and purified using [Insert purification method].
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ab100732
1
Inflammatory Biomarker Quantification in Serum
2
Measuring Coagulation Factors in Thrombosis
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The activities of MMP-9, NF-κB, tissue factor (TF), TAFI and PAI-1 in vein endothelial cells and deep venous thrombus were analyzed by commercialized kits [MMP (ab100732; Abcam), NF-κB (JK-(a)-6261; Jiangkang Bioscience, Shanghai, China), TF (ab214091; Abcam), TAFI (MA143023; Beinuo Bioscience, Shanghai, China), PAI-I (ab197752; Abcam)] and performed according to the manufacturer's instructions.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!