Anti tbx3

Manufactured by Abcam

Anti-TBX3 is a laboratory equipment product that can be used for research purposes. It is an antibody that specifically targets the TBX3 protein, which plays a role in various cellular processes. The core function of this product is to detect and measure the presence of TBX3 in biological samples.

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Lab products found in correlation

Lsm 780 confocal microscope, by Zeiss (6 mentions) Cm3050, by Leica (2 mentions) Anti hcn4, by Merck Group (2 mentions) Anti dnajb6, by Novus Biologicals (2 mentions) Apotome, by Zeiss (2 mentions) Axioplan 2 microscope, by Zeiss (2 mentions) Anti ubiquitin, by Cell Signaling Technology (1 mentions) Anti ttf1, by Abcam (1 mentions) Plx4032, by Selleck Chemicals (1 mentions) Anti usp15, by Proteintech (1 mentions) Anti ha, by Cell Signaling Technology (1 mentions) Anti gst, by Cell Signaling Technology (1 mentions) Anti gr1, by BioLegend (1 mentions) Sr11302, by Selleck Chemicals (1 mentions) Anti tpo, by Abcam (1 mentions) Anti α tubulin, by Merck Group (1 mentions) Anti ki67, by Abcam (1 mentions) Anti flag, by Merck Group (1 mentions) Sch772984, by Selleck Chemicals (1 mentions) Mg132, by Selleck Chemicals (1 mentions)

3 protocols using anti tbx3

Antibody Usage for Immunoblotting, IP, IHC, and IF

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Antibodies used for immunoblot (IB), immunoprecipitation (IP), immunohistochemistry (IHC) and immunofluorescence (IF) were as follows. Anti-TBX3 antibodies for IB Anti-TBX3 (#ab99302, Abcam, 1:500). Anti-Flag (#3165, 1:1000), Anti-α-Tubulin (T5168, 1:15000) was purchased from Sigma. Anti-HA (#3724, 1:1500), Anti-Ubiquitin (#3936, 1:500), Anti-GST (#2622, 1:1000), was purchased from Cell Signaling Technology. Anti-USP15 (#A300-923A, BETHYL, 1:1000), Anti-USP15 (#67557-1-Ig, Proteintech 1:200), Anti-Ki-67 (#ab15580, Abcam, 1:100), Anti-Tpo (#ab278525, Abcam, 1:200), Anti-Tg (#ab156008, Abcam, 1:200), Anti-Nis (#MA5-12308, Thermo, 1:400), Anti-Gr-1 (#108401, BioLegend, 1:200), Anti-PAX8 (#ab53490, Abcam, 1:10), Anti-TTF1 (#ab76013, Abcam, 1:200) were employed according to the instructions. Reagents used in this study included MG132 (#S2619, Selleck), Puromycin (#P8230, Solario), CHX (#HY-12320, MCE), Blasticidin (#B9300, Solario), PLX4032 (#S1267, Selleck), SCH772984 (#S7101, Selleck), SR11302 (#E2813, Selleck).

Zhang Z., Wu Y., Fu J., Yu X., Su Y., Jia S., Cheng H., Shen Y., He X., Ren K., Zheng X., Guan H., Rao F, & Zhao L. (2024). Proteostatic reactivation of the developmental transcription factor TBX3 drives BRAF/MAPK-mediated tumorigenesis. Nature Communications, 15, 4108.

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Immunostaining of Mouse Sinoatrial Node Tissues

Cited 1 time

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Heart samples harvested from mouse SAN tissues were embedded in a tissue freezing medium, followed by sectioning at 10 μm using a cryostat (Leica CM3050 S). The slides were subjected to immunostaining using a previously described protocol Sun et al., 2009 (link). The following antibodies were used: anti-HCN4 (Millipore, Cat#: AB5805; Novus biologicals, Cat#: NB100-74439) at 1:200, anti-DNAJB6 (Novus, Cat#: H00010049-M0; Santa Cruz Biotechnology Inc, Cat#: sc-104204) at 1:200, anti-TBX3 (abcam, Cat#: ab99302). All images were captured either using a Zeiss Axioplan II microscope equipped with ApoTome and AxioVision software (Carl Zeiss Microscopy) or a Zeiss LSM 780 confocal microscope. Signal intensity from DNAJB6 and TBX3 antibodies immunostaining was quantified using Zen 2.3 Pro software.

Ding Y., Lang D., Yan J., Bu H., Li H., Jiao K., Yang J., Ni H., Morotti S., Le T., Clark K.J., Port J., Ekker S.C., Cao H., Zhang Y., Wang J., Grandi E., Li Z., Shi Y., Li Y., Glukhov A.V, & Xu X. (2022). A phenotype-based forward genetic screen identifies Dnajb6 as a sick sinus syndrome gene. eLife, 11, e77327.

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Immunostaining of Mouse Cardiac Tissue

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Heart samples harvested from mouse SAN tissues were embedded in a tissue freezing medium, followed by sectioning at 10 μm using a cryostat (Leica CM3050 S). The slides were subjected to immunostaining using a previously described protocol. 56 The following antibodies were used: anti-HCN4 (Millipore, Cat#: AB5805) at 1:200, anti-Dnajb6 (Novus, Cat#: H00010049-M01) at 1:200, anti-Islet1 (abcam, Cat#: ab20670), anti-Tbx3 (abcam, Cat#: ab99302). All images were captured either using a Zeiss Axioplan II microscope equipped with ApoTome and AxioVision software (Carl Zeiss Microscopy) or a Zeiss LSM 780 confocal microscope.

Ding Y., Lang D., Yan J., Bu H., Li H., Jiao K., Yang J., Le T., Clark K.J., Ekker S.C., Cao H., Zhang Y., Li Y., Glukhov A.V., & Ekker S.C. (2022). A phenotype-based forward genetic screen identifiesDnajb6as a sick sinus syndrome gene.

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