Mouse anti ras

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Mouse anti-RAS is a laboratory reagent that is used for the detection and identification of the RAS protein in biological samples. It is a monoclonal antibody that specifically binds to the RAS protein, which is a key regulator of cell growth and proliferation. The Mouse anti-RAS can be used in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Mouse anti–pan-RAS antibody (3 citations) Anti-Ras (2 citations)

2 protocols using mouse anti ras

Transfection Protocols for Cancer Cell Lines

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HEK293, A375, and SW480 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS). Transient transfections were performed with TransFectin reagent (Bio-Rad, #1703352) or jetPRIME (Polyplus). Primary antibodies used were mouse anti-Rluc antibodies (Chemi-Con, #MAB4400 versus Rluc-F[2] and #MAB4410 versus Rluc-F[1]), mouse anti–HA-tag (Covance, #MMS-10P), mouse anti-FLAG (Sigma-Aldrich, #F3165), mouse anti-V5 (Invitrogen, #R9302), mouse anti-BRAF [Santa Cruz Biotechnology, sc-5284 (#F-7) and sc-166 (#C-19)], rabbit anti-CRAF (Cell Signaling Technology, #9422), mouse anti-RAS (Thermo Fisher Scientific, #MA1-012X), mouse anti-GFP (green fluorescent protein) (Roche, #11814460001), mouse anti-MEK1/2 (Cell Signaling Technology, #4684S), rabbit anti–P-MEK1/2 (Ser²¹⁷/Ser²²¹) (Cell Signaling Technology, #9154), rabbit anti–P-ERK1/2 (Cell Signaling Technology, #9101), rabbit anti-ERK1/2 (Cell Signaling Technology, #4696S), and mouse anti-PKA RIIβ (BD Biosciences, #61062).

Röck R., Mayrhofer J.E., Torres-Quesada O., Enzler F., Raffeiner A., Raffeiner P., Feichtner A., Huber R.G., Koide S., Taylor S.S., Troppmair J, & Stefan E. (2019). BRAF inhibitors promote intermediate BRAF(V600E) conformations and binary interactions with activated RAS. Science Advances, 5(8), eaav8463.

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Immunoblotting Analysis of Hippo Pathway

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Cells were harvested in EBC lysis buffer (50 mM Tris-HCl at pH 7.5, 150 mM NaCl, 5 mM EDTA, 0.5% NP-40), supplemented with Complete Mini Protease and Phosphatase Inhibitor Cocktails (Roche, Indianapolis, IN, USA). Cells were lysed and 30-80 μg protein subjected to SDS-PAGE followed by transfer onto an Immobilon-FL PVDF membrane (Millipore, Billerica, MA, USA) and incubation with the indicated antibodies. Detection was carried out with an Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA) with IR dye-tagged secondary antibodies (LI-COR Biosciences). The following antibodies were utilized: mouse anti-YAP, goat anti-NF2, mouse anti-AMOT, goat anti-AMOTL1, goat anti-AMOTL2 (Santa Cruz, Dallas, TX, USA), mouse anti-FlagM2 (Sigma, Saint Louis, MO, USA), rabbit anti-LATS1, rabbit anti-LATS2, rabbit anti-p-YAP (Cell Signaling, Danvers, MA, USA), TNKS1/2 (Santa Cruz, Dallas, TX, USA), mouse anti-TEAD4, mouse anti-RAS (Thermo Scientific, Waltham, MA, USA), mouse anti-α-Tubulin, mouse anti-β-actin (Sigma, Saint Louis, MO, USA).

Troilo A., Benson E.K., Esposito D., Garibsingh R.A., Reddy E.P., Mungamuri S.K, & Aaronson S.A. (2016). Angiomotin stabilization by tankyrase inhibitors antagonizes constitutive TEAD-dependent transcription and proliferation of human tumor cells with Hippo pathway core component mutations. Oncotarget, 7(20), 28765-28782.

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