Dulbecco’s Modified Eagle Medium nutrient mixture (DMEM) (Fisher Scientific, Grand Island, NY, USA), penicillin/streptomycin solution (pen-strep) (Fisher Scientific, Grand Island, NY, USA), fetal bovine serum (FBS) (Fisher Scientific, Grand Island, NY, USA), and amphotericin B were purchased from Invitrogen (Camarillo, CA, USA). Heparin and ketoconazole were purchased from EMD Millipore Corp. USA (Burlington MA, USA). (Sigma-Aldrich, St. Louis, MO, USA). Climbazole was purchased from Tokyo Chemical Industry Co., LTD (Toshima, Kita-Ku, Tokyo, Japan). Unless otherwise indicated, all other drugs were purchased from commercial sources.
Climbazole
Manufactured by Tokyo Chemical Industry
Sourced in Japan
Climbazole is a laboratory chemical compound produced by Tokyo Chemical Industry. It is used as an active ingredient in various industrial and research applications. The core function of Climbazole is to serve as a fungicide and antimicrobial agent.
Automatically generated - may contain errors
Lab products found in correlation
Amphotericin b, by Thermo Fisher Scientific (2 mentions)
Ketoconazole, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Heparin, by Merck Group (1 mentions)
Hydroxytamoxifen, by Merck Group (1 mentions)
Bisphenol a, by Merck Group (1 mentions)
Cell culture grade dmso, by Merck Group (1 mentions)
17α estradiol, by Merck Group (1 mentions)
Tamoxifen, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Penicillin streptomycin pen strep, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Heparin sodium salt, by Merck Group (1 mentions)
Pen strep, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Corning (1 mentions)
A549 cell, by American Type Culture Collection (1 mentions)
Penicillin streptomycin solution, by Corning (1 mentions)
3 protocols using climbazole
1
Reagents and Materials for Cell Culture
2
Screening Preservatives for Endocrine Disruption
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Preservatives triclocarban (CAS 101-20-2), triclosan (CAS 3380-34-5), bromochlorophene (CAS 15435-29-7), chlorophene (CAS 120-32-1) and climbazole (CAS 38083-17-9) were of 95% or higher purities, as specified by the manufacturer (Tokyo Chemical Industry). Control compounds (DHT; CAS 521-18-6), flutamide (FLU; CAS 13311-84-7), hydroxyflutamide (CAS 52806-53-8), (E2; CAS 50-28-2), (CAS 57-91-0), tamoxifen (CAS 10540-29-1), hydroxytamoxifen (CAS 68047-06-3), hydrocortisone (HC; CAS 50-23-7), mifepristone (CAS 84371-65-3; RU-486), dexamethasone (CAS 50-02-2), triiodothyronine (T3; CAS 6893-02-3), and bisphenol A (CAS 80-05-7) were of 97% or higher purities, as specified by the manufacturer (Sigma-Aldrich). Cell culture grade DMSO (CAS 67-68-5) was used as vehicle for chemical formulations for in vitro assays and was purchased from Sigma-Aldrich. All of the preservatives were first screened for agonist and antagonist activities in vitro at 0.1, 1.0, and (or lower, as “highest noncytotoxic,” if showed cytotoxicity). This was followed by dose-dependence assays over a broader range of concentrations if this initial screening showed endocrine-disrupting effects.
3
Cell Culture Protocol for A549 Cells
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Dulbecco’s modified Eagle medium nutrient mixture/F-12 medium (DMEM-F12) containing L-glutamine, penicillin/streptomycin solution, and Corning regular fetal bovine serum (FBS) were purchased from (Fisher Scientific, Grand Island, NY, USA), Penicillin/Streptomycin (Pen-Strep) (Fisher Scientific, Grand Island, NY, USA), and Amphotericin-B were also obtained from (Fisher Scientific, Grand Island, NY, USA). Climbazole (Tokyo Chemical Industry, Tokyo, Japan) and Heparin sodium salt (EMD Millipore Corporation, Burlington, MA, USA) were the inhibitors used in this study. Unless otherwise indicated, all other drugs were purchased from commercial sources. A549 cells were used in this study. A549 cells were obtained from (American Type Culture Collection, Manassas, VA, USA). Cells were cultured in DMEM-F12 (Fisher Scientific, Grand Island, NY, USA) supplemented with 10% FBS, 1% Pen-Strep, and 0.2% Amphotericin-B (0.5 μg/mL). For virus infection, DMEM exosome-free media was prepared with exosome-depleted FBS using DMEM/F12 medium containing L-glutamine supplemented with 2% exosome-free Corning FBS, 1% Pen-Strep, and 0.2% Amphotericin-B (0.5 μg/mL) (Fisher Scientific, Grand Island, NY, USA). Cells were cultured at 37°C in a humidified atmosphere supplemented with 5% CO2.
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