Bradford total protein concentration assay

Escitalopram oxalate, o-phthalaldehyde, reduced glutathione and Bradford reagent were from Sigma-Aldrich Chemicals GmbH (Munich, Germany), while EDTA-Na₂ and 2-thiobarbituric acid were purchased from Merck KGaA (Darmstadt, Germany). Absolute ethanol, hydrogen peroxide and n-butanol were obtained from Chimopar (Bucharest, Romania). Antibodies against BDNF, MeCP2, β actin and secondary peroxidase-linked antibodies were from Santa Cruz Biotechnology (Santa Cruz, CA, USA), while phosphorylated histone. ELISA tests for caspase-3 were purchased from Elabscience (Houston, TX, USA) and Bradford total protein concentration assay was from BioRad (Hercules, CA, USA).

Polyphenolic compounds used as references were purchased from Merck (Darmstadt, Germany) and Fluka (Saint Louis, MO, USA). Acetonitrile for the HPLC-gradient was provided by Merck (Darmstadt, Germany) and water was purified with a Direct-Q UV system by Millipore (Darmstadt, Germany). All other chemicals used were obtained from Merck (Darmstadt, Germany). Before analysis, samples were filtered through a 0.45 µm MF-Millipore™ Membrane Filter from Merck (Darmstadt, Germany). Antibodies against NF-kB p65 (Ser536) (93H1), phospho (p)NF-kB, HIF-1α, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and secondary peroxidase-linked antibodies were from Santa Cruz Biotechnology (Delaware Ave, Santa Cruz, CA, USA), while phosphorylated histone H2AX (pS139) (γH2AX) was from Stressgen Bioreagents Corporation (Victoria, BC, Canada). Finally, 2-thiobarbituric acid was obtained from Merck KGaA (Darmstadt, Germany) and Bradford total protein concentration assay was purchased from Bio-Rad (Hercules, CA, USA).