2 5 dimethoxy 4 iodoamphetamine

2,5-Dimethoxy-4-iodoamphetamine, H-89, Forskolin and BIS were obtained from Sigma-Aldrich Co., St. Louis, MO, USA. CRF, antisauvagine-30 and antalarmin were obtained from Tocris Biosciences, Ellisville, MO, USA. Tat-CRFR₁-CT peptide was obtained from CanPeptide, Inc., Pointe-claire, QC, Canada. The Tat-CRFR₁-CT sequence used was YGRKKRRQRR-PTRVSFHSIKQSTAV. This peptide has been shown previously to prevent the functional synergism between CRFR₁ and 5-HT₂Rs (Magalhaes et al., 2010 (link)). Di-4-ANEPPS stock solution was prepared in alcohol and cremophore-EL solution which can be stored at 4°C for 2 months. FBS and ACSF were added on the day of experiment. CRF stock was dissolved in HBSS and milli-Q water mixture. DOI was prepared in alcohol and Milli-Q water. Forskolin, H-89, BIS, antalarmin, antisauvagine-30, and PMA stock solutions were prepared in dimethyl sulfoxide (DMSO). All the stock solutions were made 1000 times more concentrated than working concentrations.