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Oxiselect intracellular ros rns assay kit

Manufactured by Cell Biolabs

The OxiSelect™ Intracellular ROS/RNS Assay kit is a fluorometric assay designed to detect and quantify intracellular reactive oxygen species (ROS) and reactive nitrogen species (RNS) in cells. The kit utilizes a cell-permeable fluorogenic probe that becomes fluorescent upon oxidation by ROS/RNS.

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3 protocols using oxiselect intracellular ros rns assay kit

1

Intracellular ROS/RNS Quantification

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The RONS level was assessed by OxiSelect™ Intracellular ROS/RNS Assay kit (Cell Biolabs, Inc., San Diego, CA). For this purpose, cell lysate was used and preparation of this based on Kit instructions.
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2

Intracellular ROS/RNS Measurement

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The RONS level was assessed by OxiSelect™ Intracellular ROS/RNS Assay kit (Cell Biolabs, Inc., San Diego, CA), following the protocol.
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3

Oxidative Stress and Transcription Factors

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The level of ROS and RNS were determined in tissue lysate samples by the OxiSelect™ Intracellular ROS/RNS Assay Kit (Cell Biolabs, Inc., San Diego, CA), based on the kit protocol.
Quantification of NF-kB, Rb and p53 was performed in the tissue lysates by NF-kB p65 Transcription Factor Assay® Kit, Human Retinoblastoma ELISA® Kit (Sigma-Aldrich, Saint Louis, USA) and Abcam’s p53 Simple Step ELISA® Kit (Cambridge, USA), respectively, based on the kit protocols.
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