Hybridization of arrays (GeneChip Mouse Exon 1.0 ST Array, Affymetrix, Santa Clara, CA) was performed in duplicate using independent biological samples. Affymetrix GeneChip Analysis Suite software (MAS 5.0) was used to generate raw data and genes with normalized expression levels over detection threshold were called and analyzed for differential expression using the Cyber T program (Long et al., 2001 (link)) (http://cybert.ics.uci.edu/ ). The microarray data have been deposited into the GEO database (accession number GSE55075).
Genechip analysis suite software mas 5.0
Manufactured by Thermo Fisher Scientific
The GeneChip Analysis Suite software (MAS 5.0) is a comprehensive software package designed for the analysis of gene expression data generated using Affymetrix GeneChip microarray technology. The software provides a suite of tools for data preprocessing, normalization, and statistical analysis of gene expression levels.
Automatically generated - may contain errors
2 protocols using genechip analysis suite software mas 5.0
1
Microarray Analysis of Differential Gene Expression
2
Genome-Wide Transcriptome Analysis of Skin
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Total RNA was extracted from skin using TRIzol reagent (Invitrogen) according to the manufacturer's instructions. One μg of total RNA was reverse transcribed into cDNA using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems) according to the manufacturer's instructions. Hybridization of arrays (GeneChip Mouse Exon 1.0 ST Array; Affymetrix) was performed in duplicate using independent biological samples. Affymetrix GeneChip Analysis Suite software (MAS5.0) was used to generate raw data, and genes with normalized expression levels over detection threshold were called and analyzed.
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