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2 protocols using fc reagent

1

Phytochemical Analysis and Antioxidant Assays

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Gallic acid (C7H6O5), FC reagent (C6H6O), ABTS (C18H18N4O6S4), Ellman’s reagent (C14H8N2O8S2), sodium carbonate (Na2CO3), 1,1-diphenyl-2-picrylhydrazyl (C18H12N5O6), ascorbic acid (C6H8O6), aluminum chloride hexahydrate (AlCl3·6H2O), sulfuric acid (H2SO4), ferric chloride (FeCl3), bovine serum albumin, lysogeny broth, ethanol, and nutrient agar were purchased from Hi-Media (Mumbai, India). Acetylcholinesterase and silver nitrate were purchased from Sigma (Roeder mark, Germany). Using a spectrophotometer (Shimadzu UV-1601, Tokyo, Japan), calorimetric analysis was carried out.
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2

Antimicrobial and Antioxidant Assay of Chitosan

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Low-molecular-weight chitosan, glycerol, methanol, FC reagent, DPPH (2,2-diphenyl-1-picrylhydrazyl), TPTZ (2,4,6-tripyridyl-s-triazine), sodium carbonate, gallic acid, quercetin, Muller–Hinton agar, aluminum chloride, and potassium acetate were supplied by Hi-Media and Hi-Tech chemicals (New Delhi, India). The bacterial strain (E. coli- NCDC 134) was provided by the National Dairy Research Institute, Karnal (India).
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