EBM-2 Basal Medium and EGM-2 SingleQuot Kit supplement/growth factors were purchased from Lonza Walkersville, Inc. (Walkersville, MD), and EGM Cell Growth Medium-2 was prepared according to the manufacturer’s instructions. NRG-1 (ECD, 377-HB) and EGF (236-EG) were purchased from Bio-Techne/R&D Systems. TAK-165 and GI 254023X were obtained from Tocris Bioscience (Bristol, UK) and diluted in dimethyl sulfoxide (Cell Culture grade, Sigma). Collagenase II (345 units per mg, CLS-2) was purchased from Worthington Biochemical Corporation (Lakewood, NJ), dispase II (04942078001) was from Roche Life Science (Indianapolis, IN). D-glucose and DNase I was from Sigma (St. Louis, MO). For experiments involving cell stimulation, final concentrations of dimethyl sulfoxide did not exceed 0.1%.
D glucose and dnase 1
Manufactured by Merck Group
D-glucose is a monosaccharide, a simple sugar that serves as a primary source of energy for many organisms. DNase I is an enzyme that catalyzes the hydrolytic cleavage of DNA.
Automatically generated - may contain errors
Lab products found in correlation
Cell culture grade, by Merck Group (2 mentions)
Ebm 2 basal medium, by Lonza (2 mentions)
Egf 236 eg, by R&D Systems (2 mentions)
Tak 165, by Bio-Techne (2 mentions)
Gi254023x, by Bio-Techne (2 mentions)
Egm 2 singlequot kit, by Lonza (2 mentions)
Dispase 2, by Roche (2 mentions)
Collagenase 2, by Worthington (2 mentions)
2 protocols using d glucose and dnase 1
1
Isolation and Culture of Endothelial Cells
2
Endothelial Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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EBM-2 Basal Medium and EGM-2 SingleQuot Kit supplement/growth factors were purchased from Lonza Walkersville, Inc. (Walkersville, MD), and EGM Cell Growth Medium-2 was prepared according to the manufacturer's instructions. NRG-1 (ECD, 377-HB) and EGF (236-EG) were purchased from Bio-Techne/R&D Systems. TAK-165 and GI 254023X were obtained from Tocris Bioscience (Bristol, UK) and diluted in dimethyl sulfoxide (Cell Culture grade, Sigma). Collagenase II (345 units per mg, CLS-2) was purchased from Worthington Biochemical Corporation (Lakewood, NJ), dispase II (04942078001) was from Roche Life Science (Indianapolis, IN). D-glucose and DNase I was from Sigma (St. Louis, MO). For experiments involving cell stimulation, nal concentrations of dimethyl sulfoxide did not exceed 0.1%.
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