Ino80

The total protein was extracted from CMs with RIPA lysis buffer (Beyotime, China), and a BCA assay (Beyotime, China) was performed to quantify the protein concentrations. The equal amount of protein samples was loaded and resolved on SDS-PAGE gel, and transferred to nitrocellulose membrane (Millipore, USA). The membranes were blocked in 5% milk and then reacted overnight with primary antibodies against Ino80, Dis3, Srsf1 and GAPDH (Proteintech, USA) at 4 °C. The membranes were finally incubated with secondary antibodies for 1 h at RT and scanned with Odyssey (LI-COR Biosciences, USA).

Cultured cells were lysed in strong RIPA buffer containing Halt Protease Inhibitor Cocktails (Thermo, Waltham, MA). Protein concentrations were measured using a BCA protein assay kit (Pierce, Rockford, IL). Primary antibodies targeting NANOG (Abcam), Ino80 (proteintech, Chicago, IL), OCT4 (Santa Cruz Biotechnology, Santa Cruz, CA), p53 (Santa Cruz Biotechnology), BAX (Abcam) and GAPDH (Santa Cruz Biotechnology) were incubated with the proteins overnight at 4°C, followed by incubation with the appropriate HRP (horseradish peroxidase)-conjugated secondary antibodies. Detection of HRP was performed using the Super Signal West Pico Chemiluminescent Substrate (Pierce).