Anti s6

Manufactured by Merck Group

Anti-S6 is a laboratory equipment product designed for the detection and analysis of the S6 protein. It serves as a tool for researchers and scientists studying cellular processes and signaling pathways involving the S6 protein. The core function of Anti-S6 is to provide a reliable and specific method for the identification and quantification of the S6 protein in various biological samples.

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Lab products found in correlation

Anti β actin, by MP Biomedicals (1 mentions) Anti s6k, by Merck Group (1 mentions) Anti β actin, by Santa Cruz Biotechnology (1 mentions) Anti akt, by Merck Group (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Anti p aktser473, by Merck Group (1 mentions) Anti tsc1, by Cell Signaling Technology (1 mentions) Anti myc, by Merck Group (1 mentions) Western lightning plus ecl, by PerkinElmer (1 mentions) Anti p plcγ2, by Cell Signaling Technology (1 mentions) Odyssey infrared imaging system software, by LI COR (1 mentions) Anti tubulin, by Merck Group (1 mentions) Anti ha, by Fortrea (1 mentions)

2 protocols using anti s6

Immunoblotting Detailed Protocol

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Immunoblotting was performed as described earlier (Zidek et al, 2015) with the following antibodies: from Cell Signaling Technology, anti‐TSC1 (#4906), anti‐TSC2 (#4308); anti‐pS6K (Thr389) (#9206) and (Thr389) (#9234), anti‐S6K (#9202), anti‐pS6 (Ser235/236) (#2211), anti‐S6 (#2317), anti‐pSAPK/JNK (Thr183/Tyr185) (#9251); anti‐p‐4E‐BP1 (T37/46) (#9459); anti‐p‐AKT (Ser473) (#4060); anti‐AKT (#9272); anti‐MYC (#5605); from Sigma‐Aldrich, anti‐β‐actin (A3853); from Santa Cruz Biotechnology, anti‐α‐tubulin (sc‐8035), anti‐TSC2 (sc‐893), anti‐MYC (sc‐40), anti‐SAPK/JNK (sc‐571); and from MP Biomedicals, anti‐β‐actin (#69100). Bands were visualized by chemoluminescence (Western Lightning Plus‐ECL, Perkin Elmer).

Hartleben G., Müller C., Krämer A., Schimmel H., Zidek L.M., Dornblut C., Winkler R., Eichwald S., Kortman G., Kosan C., Kluiver J., Petersen I., van den Berg A., Wang Z, & Calkhoven C.F. (2018). Tuberous sclerosis complex is required for tumor maintenance in MYC‐driven Burkitt's lymphoma. The EMBO Journal, 37(21), e98589.

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Protein Extraction and Immunoblotting Assay

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Whole-cell protein extracts were prepared from Ramos B cell lines by cell lysis with buffer containing 50mM Tris (pH 7.4), 150mM sodium chloride, 1mM EDTA, 1% Triton X-100, 1mM sodium orthovanadate, 0.25% sodium deoxycholate, and protease inhibitors. Cell lysates were cleared by centrifugation and were separated by SDS-PAGE under reducing conditions. Following electrophoretic transfer, nitrocellulose membranes were blocked, probed with primary Ab, followed by IRDye-labeled secondary Abs (LI-COR Biosciences). Antibody binding was detected and quantified using the Odyssey infrared imaging system software (LI-COR Biosciences). Abs used for immunoblot analysis included anti-pPLCγ2 (Y1217, Cell Signaling Technology (CST)), anti-PLCγ2 (CST), anti-pS6 (S235/236, CST), anti-S6 (CST), anti-tubulin (Sigma), anti-pY100 (pan pY, CST), and anti-HA (Covance).

Dam E.M., Habib T., Chen J., Funk A., Glukhova V., Davis-Pickett M., Wei S., James R., Buckner J.H, & Cerosaletti K. (2016). THE BANK1 SLE-RISK VARIANTS ARE ASSOCIATED WITH ALTERATIONS IN PERIPHERAL B CELL SIGNALING AND DEVELOPMENT IN HUMANS. Clinical immunology (Orlando, Fla.), 173, 171-180.

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