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2 protocols using o phthalaldehyde opa

1

Comprehensive Lemon Metabolite Analysis

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Fresh lemons (Eureka, Anyue, Sichuan, China) were purchased from a local supermarket in Nanjing. A standard sample of Cyclohexanone (>99.9%; C6–C25) was purchased from Sigma-Aldrich Trading Co., Ltd. (Shanghai, China). Sodium chloride, amino acid standard, o-phthalaldehyde (OPA), acetonitrile, methanol, 3-mercaptopropionic acid, boric acid, potassium dihydrogen phosphate, petroleum ether (30–60 °C), absolute ethanol, phosphoric acid, hydrochloric acid, ammonia water, ether, pyrogallic acid, ethanol (95%), and other reagents were purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China).
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2

Quantification of Carbohydrates using OPA

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D-(+)-glucose (C6H12O6, MW 180.16), L-(+)-arabinose (C5H10O5, MW 150.13), and D-(+)-xylose (C5H10O5, MW 150.13) (All 99% purity) were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). O-phthalaldehyde (OPA), disodium hydrogen phosphate, sodium dihydrogen phosphate, methanol, sodium dodecyl sulfate (SDS), borax, and β-mercaptoethanol were of analytical reagent grade and supplied by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China).
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