Insulin

Manufactured by AstraZeneca

Insulin is a lab equipment product used to measure and analyze insulin levels. It is a hormone produced by the pancreas that regulates blood sugar levels. The core function of this product is to provide accurate and reliable measurements of insulin concentration in biological samples.

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Lab products found in correlation

Pioglitazone, by AstraZeneca (2 mentions) Transferrin alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Egf alexa fluor 488, by Thermo Fisher Scientific (1 mentions) Formaldehyde, by Merck Group (1 mentions) Cholesterol, by Merck Group (1 mentions) Digitonin, by Merck Group (1 mentions) Dmpe peg2000, by NOF corporation (1 mentions) Penicillin and streptomycin, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Fbs superior, by Merck Group (1 mentions) 3 isobutyl 1 methyxanthine, by Merck Group (1 mentions)

2 protocols using insulin

Liposome Delivery of Fluorescent-Labeled Biomolecules

Cited 1 time

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FBS Superior, dexamethasone, 3-isobutyl-1-methyxanthine, insulin, and pioglitazone were provided by Astra Zeneca. P/S, transferrin Alexa Fluor 568 (T23365; Invitrogen), EGF Alexa Fluor 488 (E13345; Invitrogen), LDL-pHrodo Red (L34356; Invitrogen), LDL-Alexa Fluor 488 (homemade) were as previously reported (Franke et al., 2019 (link)). pH calibration buffers were from Invitrogen (P35379). LNPs were prepared using the cationic ionizable lipids MC3, L608, ACU5, ACU22, MOD5, and L319 (AstraZeneca) and the helper lipids cholesterol (Sigma-Aldrich), 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC; CordenPharma), 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine-N [methoxy (polyethylene glycol)-2000] (DMPE-PEG2000; NOF Corporation), and contained CleanCap eGFP mRNA (5-methoxyuridine, cat. no. L-7201) and/or CleanCap Cy5 eGFP mRNA (5-methoxyuridine, batch no. WOTL18871, cat. no. L-7701; TriLink Bio Technologies). Formaldehyde was from Merck and digitonin from Sigma-Aldrich.

Paramasivam P., Franke C., Stöter M., Höijer A., Bartesaghi S., Sabirsh A., Lindfors L., Arteta M.Y., Dahlén A., Bak A., Andersson S., Kalaidzidis Y., Bickle M, & Zerial M. (2021). Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale. The Journal of Cell Biology, 221(2), e202110137.

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Lipid Nanoparticle Preparation and Characterization

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FBS Superior (Merck, S0615), dexamethasone (Sigma Aldrich, D2915), 3-isobutyl-1methyxanthine (Sigma Aldrich, I5879). Insulin (Actrapid Novonordisk) and Pioglitazone (AZ10080838) are provided by Astra Zeneca. Penicillin and Streptomycin (Gibco, 15140-122), Transferrin Alexa-568 (Invitrogen™, T23365), EGF Alexa-488 (Invitrogen™, E13345), LDL-pHRodo (Invitrogen ™, L34356), LDL-Alexa 488 was homemade, as previously reported (Franke, Repnik et al. 2019) . pH calibration buffers (Invitrogen™, P35379). LNP were prepared using the cationic

Paramasivam P., Franke C., Stöter M., Höijer A., Bartesaghi S., Sabirsh A., Lindfors L., Arteta M.Y., Dahlén A., Bak A., Andersson S., Kalaidzidis Y., Bickle M., & Zerial M. (2020). Endosomal escape of delivered mRNA from endosomal recycling tubules visualized at the nanoscale.

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