Guanosine triphosphate gtp

Poly (acrylic acid) (PAA, MW ~5000) was purchased from Ryon Biological Technology (Shanghai, China). Polyethyleneimine (PEI, MW ~10,000) was purchased from Damas-beta. Tris (hydroxymethyl) aminomethane (Tris) was purchased from Alfa Aesar. KCl, NaCl, and MgCl₂ were obtained from Aladdin reagent (Shanghai, China). Adenosine 5′-triphosphate (ATP) disodium salt solution, uridine Triphosphate (UTP), cytidine triphosphate (CTP) and guanosine triphosphate (GTP) were purchased from Sigma-Aldrich. HeLa and PC3 cells were obtained from Chinese Center for Typical Culture Collection (Wuhan, China) and cultured in DMEM (Gibco) supplemented with 10% (v/v) fetal bovine serum (FBS), 2 mg/mL NaHCO₃, and 100 U/mL antibiotics 15 (penicillin-streptomycin). MCF-7 cells were purchased from KeyGEN Biotech Co. Ltd. (Nanjing, China) and cultured in RPMI-1640 (Gibco) supplemented with 10% FBS, 2 mg/mL NaHCO₃, and 100 U/mL antibiotics (penicillin–streptomycin) at 37 °C in a humidified 5% CO₂ atmosphere. AAO membranes were purchased from Pu-Yuan Nano Technology Co. Ltd. (Hefei, China). The thickness of AAO membrane is 65 μm. All solutions were prepared using Millipore Milli-Q water (18 MΩ cm). All oligonucleotides are synthesized and purified by Sango Biotech Co. Ltd, (Shanghai, China). The sequences are shown in Tables S2 and S3.