Calcium chloride cacl2

The telomeric DNA strands (RevHumTel and HumTel), unmodified and modified with organic dyes, have been acquired from Metabion International AG (Planegg, Germany) (HPLC purified, dissolved in water). All DNA strands have been used as delivered without further treatment. Magnesium chloride (MgCl₂, ≥98 %), sodium chloride (NaCl) and Tris acetate-EDTA buffer (TAE buffer, 10× concentrated) have been acquired from Sigma Aldrich (Hamburg, Germany). The diluted TAE buffer (1× concentrated, in ultrapure water (Merck KGaA, Darmstadt, Germany)) (pH = 8.2) contains 40 mM Tris-acetate and 1 mM EDTA. Calcium chloride (CaCl₂, ≥98 %) has been purchased from Carl Roth (Karlsruhe, Germany) and potassium chloride (KCl) from VWR Prolabo (Darmstadt, Germany). The salt solutions have been prepared in ultrapure Millipore water.

Maltodextrin, with a dextrose equivalent (DE) of 2 and a molecular weight Mw of ~124 kDa (MD DE2) (ElianeTM MD2 by Avebe, Veendam, The Netherlands), maltodextrin DE 21 (Glucidex^® 21D by Roquette, Lestrem, France) (MD DE21), and potato protein (PP) (Solanic^® 300 by Avebe, Veendam, The Netherlands), with a protein content of 91.16 ± 0.3 g/100 g, were used. The chemicals ortho-phtaldialdehyde (OPA) (purity ≥ 99%), calcium chloride CaCl₂ (≥98%), trichloroacetic acid (≥99%), and sodium dodecyl sulfate (SDS) (≥99%) were purchased from Carl Roth GmbH & Co. KG, (Karlsruhe, Germany), along with TRIS-buffer from AppliChem GmbH (Darmstadt, Germany), and ethanol (≥99.8%), β-mercaptoethanol (≥98%), L-lysine, azocasein, bovine pancreatic trypsin, sodium hydroxide (NaOH), and hydrochloric acid (HCl) from Sigma-Aldrich Chemie GmbH (Steinheim, Germany), as well as 0.1 M sodium tetraborate buffer (pH 9.7) from Merck KGaA (Darmstadt, Germany).