NCI-N87 and NCI-N87-R cells were cultured in six-well plates. The confluent cell monolayers were wounded by scraping once horizontally and vertically with a 200 μl pipette tip and further incubated in DMEM with 0.5% FBS. The images of the wounds at different time points were captured using the Olympus CKX41 microscope system.
Ckx41 microscope system
Manufactured by Olympus
The CKX41 microscope system is a versatile and compact inverted microscope designed for a wide range of laboratory applications. It features high-quality optics, a stable and ergonomic design, and various user-friendly features for efficient observation and documentation of samples.
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Lab products found in correlation
4 protocols using ckx41 microscope system
1
Wound Healing Assay for NCI-N87 Cells
2
3D Cell Culture Imaging Protocol
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3D cell cultures were performed as described in the previous study [55 (link)]. Briefly, NCI-N87 and NCI-N87-R cells (1 × 105/ml) were suspended in a mixture of Matrigel matrix (BD-Biosciences) and culture medium (1:24, v/v) and then layered onto solidified Matrigel. After one week incubation, the images of the cells were captured using the Olympus CKX41 microscope system.
3
Wound Healing Assay Protocol
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Cells were plated in a monolayer with more than 90% fusion. A pipette tip was utilized to draw straight and evenly scratches in each well. Cells were rinsed with PBS. The culture solution was replaced with DMEM containing low serum (0.5% FBS) for each well, and SMY002 was added. Wound healing was observed and photographed with an Olympus CKX41 microscope system.
4
Migratory and Invasive Ability Assay
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For migratory ability assay, 2×10 5 cells suspended with 100 µl DMEM were added to the upper chambers (8 µm pore sizes, Corning, US) and supplemented with or without SMY002. For invasive ability assay, the upper chamber was coated with 100 µl Matrigel (1 mg/ml, BD, US) prior to seed cells. 800 µl of DMEM containing 20% FBS was added into the lower chambers. Cultured for 48 h, cells were xed with methanol for 30 min and stained with 0.1% crystal violet for 30 min at room temperature. The inserts were immersed twice with PBS. The membranes were photographed using an Olympus CKX41 microscope system and counted by ImageJ software.
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