Hca standards of iq

Fresh chicken breast was purchased from a local market (Zhanjiang, Guangdong province, China), cleaned, removed the fascia, and minced using a grinder. Cyanidin-3-O-glucose (C3G) with a purity of 98% was ordered from Durst Biotechnology Co., LTD (Chengdu, China). HCA standards of IQ、MeIQ、MeIQx、4,8-DiMeIQx、NorHarman、Harman、PhIP、AαC、MeAαC、Trp-P-2、Trp-P-1A were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Chromatographic grade of methanol, acetonitrile, ammonium acetate, triethylamine, phosphoric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate and other analytical grade reagents were bought from Sinopharm chemical reagent Co., LTD (Beijing, China). Methyl cinnamate and 4A molecular sieve of analytical grade were ordered from Maclin Biochemical Technology Co., LTD (Shanghai, China). Novozym 435 lipase was purchased from Novozymes Biotechnology Co., LTD (Shenyang, China).

Cyanidin-3-O-glucose (C3G) with a purity of 98 % was ordered from Durst Biotechnology Co., Ltd (Chengdu, China). HCA standards of IQ, MeIQx, 4,8-DiMeIQx, NorHarman, Harman, PhIP, and AαC were purchased from Santa Cruz Biotechnology Inc (Santa Cruz, CA, USA). Chromatographic grade of methanol, acetonitrile, ammonium acetate, triethylamine, phosphoric acid, sodium dihydrogen phosphate, disodium hydrogen phosphate and other analytical grade reagents were brought from Sinopharm chemical reagent Co., Ltd (Beijing, China). Methyl cinnamate and 4A molecular sieve of analytical grade were ordered from Maclin Biochemical Technology Co., Ltd (Shanghai, China). Novozym 435 lipase was purchased from Novozymes Biotechnology Co., Ltd (Shenyang, China).