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Cd45rb fitc

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CD45Rb-FITC is a fluorescently-labeled antibody that binds to the CD45Rb isoform of the CD45 protein expressed on the surface of certain immune cells. It is used in flow cytometry applications to identify and quantify these cell populations.

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Lab products found in correlation

Cd4 apc, by Thermo Fisher Scientific (1 mentions) Mouse cd4 t cell isolation kit, by STEMCELL (1 mentions) Cd62l pe, by Thermo Fisher Scientific (1 mentions) Lsrii flow cytometer, by BD (1 mentions) Foxp3 transcription factor staining buffer set, by Thermo Fisher Scientific (1 mentions) Foxp3 apc, by Thermo Fisher Scientific (1 mentions) Cyan adp analyzer, by Beckman Coulter (1 mentions) Cd11b pacific blue, by Thermo Fisher Scientific (1 mentions) Cd138 apc, by Thermo Fisher Scientific (1 mentions) B220 pe texasred, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

CD45RB (3 citations) Anti-CD45RB FITC (2 citations)

2 protocols using cd45rb fitc

1

Naïve CD4+ T Cell Transfer Colitis

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Naïve CD4+ T cell were isolated from C57BL/6 (CD45.1) mouse spleens and lymph nodes with a mouse CD4+ T cell isolation kit as per the manufacturer's instructions (Stemcell Technology, Canada). The isolated CD4+ T population was labeled with CD45Rb-FITC, CD62L-PE, and CD4-APC (eBiosciences, San Diego, CA). CD4+CD62L+CD45RBhi T cells were sorted by flow cytometry. Then 3.5 × 105 cells were injected into RAG1−/− mice by intraperitoneal (IP) injection. Mouse body condition, the form of their stool and weight were monitored at least weekly. Mice started to lose weight and develop looser stools around 3–4 weeks in our facility indicating that colitis was present.
Chen X., Berin M.C., Gillespie V.L., Sampson H.A, & Dunkin D. (2021). Treatment of Intestinal Inflammation With Epicutaneous Immunotherapy Requires TGF-β and IL-10 but Not Foxp3+ Tregs. Frontiers in Immunology, 12, 637630.
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2

Multiparameter Flow Cytometry of Murine Immune Cells

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The anti-mouse antibodies B220-PE-Texas Red, CD4-APC-CyC7, CD8-PerCP-Cy7, Foxp3-APC, CD11b–Pacific Blue, CD45RB –FITC, CD138-APC, IgA-FITC, anti-α4β7-FITC and CCR9-PerCP-Cy7 and their respective fluorochrome-conjugated isotypes were purchased from eBioscience (San Diego, CA). Intracellular Foxp3 staining was performed using the Foxp3-Transcription Factor Staining Buffer Set (eBioscience, San Diego, CA) as per manufacturer’s recommended protocol. Cells were acquired on an LSRII flow cytometer (BD Biosciences, San Diego, CA) or a CyAn™ ADP Analyzer (Beckman, Coulter, Inc., Carlsbad, CA) and data were analyzed using FlowJo software (Tree Star, Ashland, Or).
Wang L., Ray A., Jiang X., Wang J.Y., Basu S., Liu X., Qian T., He R., Dittel B.N, & Chu Y. (2015). T regulatory cells and B cells cooperate to form a regulatory loop that maintains gut homeostasis and suppresses dextran sulfate sodium-induced colitis. Mucosal Immunology, 8(6), 1297-1312.
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