Cellular lysates in RIPA lysis buffer (ThermoFisher Scientific) were electrophoresed using the XCell SureLock Mini-Cell Electrophoresis System with a NuPAGE Novex 3–8% acetate gel (ThermoFisher Scientific). Proteins were transferred to a nitrocellulose membrane and stained with the 4D2 mouse monoclonal anti-human collagen 7 antibody (Santa Cruz Biotechnology, Dallas, TX, USA). Secondary staining was performed with a goat anti-mouse horseradish peroxidase conjugated antibody (Santa Cruz Biotechnology) and detection with the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).
Nupage novex 3 8 acetate gel
Manufactured by Thermo Fisher Scientific
The NuPAGE Novex 3–8% acetate gel is a pre-cast polyacrylamide gel designed for the electrophoretic separation of proteins. It has a gradient of 3–8% acrylamide concentration, which allows for the effective separation of a wide range of protein molecular weights.
Automatically generated - may contain errors
Lab products found in correlation
Ripa lysis buffer, by Thermo Fisher Scientific (2 mentions)
Horseradish peroxidase conjugated goat anti mouse antibody, by Santa Cruz Biotechnology (2 mentions)
Xcell surelock mini cell electrophoresis system, by Thermo Fisher Scientific (2 mentions)
4d2 mouse monoclonal anti human collagen 7 antibody, by Santa Cruz Biotechnology (2 mentions)
Supersignal west pico chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions)
2 protocols using nupage novex 3 8 acetate gel
1
Collagen 7 Protein Detection in Cell Lysates
2
Collagen 7 Protein Detection in Cell Lysates
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Cellular lysates in RIPA lysis buffer (ThermoFisher Scientific) were electrophoresed using the XCell SureLock Mini-Cell Electrophoresis System with a NuPAGE Novex 3–8% acetate gel (ThermoFisher Scientific). Proteins were transferred to a nitrocellulose membrane and stained with the 4D2 mouse monoclonal anti-human collagen 7 antibody (Santa Cruz Biotechnology, Dallas, TX, USA). Secondary staining was performed with a goat anti-mouse horseradish peroxidase conjugated antibody (Santa Cruz Biotechnology) and detection with the SuperSignal West Pico Chemiluminescent Substrate (ThermoFisher Scientific).
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