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Prussian blue

Manufactured by ScyTek Laboratories
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Sourced in Germany

Prussian blue is a chemical compound that has the chemical formula Fe7(CN)18. It is a dark blue crystalline solid that is widely used as a pigment and in various industrial applications. Prussian blue is known for its intense blue color and its ability to absorb and reflect light.

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Lab products found in correlation

Ab10558, by Abcam (1 mentions) May gr nwald giemsa stain, by Merck Group (1 mentions)

Close spelling variants of this product

Prussian blue reaction

2 protocols using prussian blue

1

Histological Analysis of Cardiac and Pulmonary Fibrosis

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60 to 69 days after immunization, animals were sacrificed and heart and lungs were fixed in 10% neutral buffered formalin, embedded in paraffin, sectioned at 6 μm, and stained with picrosirius or Prussian blue (ScyTek) following manufacturer's recommendations. The degree of fibrosis was graded by an experienced pathologist on picrosirius stained sections under white light; density of alveolar siderophages in lung parenchyma was quantified using semiautomated routine in Fiji [13 (link)]. For CD45 immunohistochemistry, reagents from Dako Ltd. were used. Heat-mediated antigen retrieval was performed. Dakoreal™ EN Vision TM Detection System Peroxidase/DAB was used to visualize the signal. Rabbit polyclonal anti-CD45 antibody (Abcam ab10558) was used at 1 : 1000 dilution. CD45 positive foci were quantified by counting in 10 random fields (magnification 400x).
Bogomolovas J., Šimoliūnas E., Rinkūnaitė I., Smalinskaitė L., Podkopajev A., Bironaitė D., Weis C.A., Marx A., Bukelskienė V., Gretz N., Grabauskienė V., Labeit D, & Labeit S. (2016). A Novel Murine Model of Parvovirus Associated Dilated Cardiomyopathy Induced by Immunization with VP1-Unique Region of Parvovirus B19. BioMed Research International, 2016, 1627184.
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2

Cytospin Staining for Iron-Rich Erythroblasts

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Cytospin preparation and staining with May–Grünwald–Giemsa stain (Merck KgaA, Darmstadt, Germany) or Prussian blue (ScyTek Laboratories, Inc., West Logan, UT) were performed as described previously25 (link). RS was defined as erythroblasts with ≥ five iron granules surrounding at least one-third of the nuclear lesion55 (link).
Ochi T., Fujiwara T., Ono K., Suzuki C., Nikaido M., Inoue D., Kato H., Onodera K., Ichikawa S., Fukuhara N., Onishi Y., Yokoyama H., Nakamura Y, & Harigae H. (2022). Exploring the mechanistic link between SF3B1 mutation and ring sideroblast formation in myelodysplastic syndrome. Scientific Reports, 12, 14562.
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