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Standard forceps type circular electrodes

Manufactured by Nepa Gene
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Sourced in Japan

The standard forceps-type circular electrodes are a versatile lab equipment designed for various applications. These electrodes feature a circular shape and are operated using a forceps-like mechanism. They are intended for use in controlled laboratory settings, but their core function is to establish electrical connections for measurement or stimulation purposes, without further interpretation of their intended use.

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Lab products found in correlation

Fast green, by Merck Group (2 mentions) Pcag ires egfp, by Merck Group (2 mentions) Cuy21edit, by Nepa Gene (2 mentions)

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Forceps-type electrodes

2 protocols using standard forceps type circular electrodes

1

Tripolar In Utero Electroporation of Visual Cortex

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tripolar in utero electroporation of the visual cortex was performed as previously 52 (link). Timed-pregnant Long Evans rats were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (pCAG-IRES-EGFP, 1.5 μg/μL in water) and dye Fast Green (0.3 μg/μL; Sigma, St. Louis, MO) were injected (5–6 μL) through the uterine wall into one of the embryos’ lateral ventricles by a 30-G needle. While the embryo’s head was carefully held between standard forceps-type circular electrodes (10 mm diameter; positive poles; Nepa Gene, Chiba, Japan), a third electrode (7×6×1 mm, negative pole) was positioned on the back of the embryo’s head. 5 electrical pulses (amplitude, 50V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Deidda G., Allegra M., Cerri C., Naskar S., Bony G., Zunino G., Bozzi Y., Caleo M, & Cancedda L. (2014). Early depolarizing GABA controls critical period plasticity in the rat visual cortex. Nature neuroscience, 18(1), 87-96.
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2

Tripolar In Utero Electroporation of Visual Cortex

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tripolar in utero electroporation of the visual cortex was performed as previously 52 (link). Timed-pregnant Long Evans rats were anaesthetized at E17.5 with isoflurane (induction, 3.5%; surgery, 2.5%), and uterine horns were exposed by laparotomy. Expression vectors (pCAG-IRES-EGFP, 1.5 μg/μL in water) and dye Fast Green (0.3 μg/μL; Sigma, St. Louis, MO) were injected (5–6 μL) through the uterine wall into one of the embryos’ lateral ventricles by a 30-G needle. While the embryo’s head was carefully held between standard forceps-type circular electrodes (10 mm diameter; positive poles; Nepa Gene, Chiba, Japan), a third electrode (7×6×1 mm, negative pole) was positioned on the back of the embryo’s head. 5 electrical pulses (amplitude, 50V; duration, 50 ms; intervals, 100 ms) were delivered with a square-wave electroporation generator (CUY21EDIT; Nepa Gene). Uterine horns were returned into the abdominal cavity, and embryos continued their normal development.
Deidda G., Allegra M., Cerri C., Naskar S., Bony G., Zunino G., Bozzi Y., Caleo M, & Cancedda L. (2014). Early depolarizing GABA controls critical period plasticity in the rat visual cortex. Nature neuroscience, 18(1), 87-96.
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