One million MC38 or hIL-15-MC38 cells were incubated in 5 mL of medium at 37 °C for 72 hours; then supernatants were collected. MC38 or hIL-15-MC38 tumor-bearing mice were euthanized 14 days after inoculation. Tumors were harvested, and tumor homogenates were prepared. Peripheral blood samples were collected from mice, and plasma was prepared. Human IL-15 concentration was measured using Human IL-15 DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol.
Human il 15 duoset elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human IL-15 DuoSet ELISA kit is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human interleukin-15 (IL-15) levels in cell culture supernates, serum, and plasma.
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3 protocols using human il 15 duoset elisa kit
1
Quantifying Tumor-Derived Human IL-15
2
Quantification of Human IL-15 in MC38 Tumor Model
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One million MC38 or hIL-15-MC38 cells were incubated in 5 mL of medium at 37 °C for 72 hours; then supernatants were collected. MC38 or hIL-15-MC38 tumor-bearing mice were euthanized 14 days after inoculation. Tumors were harvested, and tumor homogenates were prepared. Peripheral blood samples were collected from mice, and plasma was prepared. Human IL-15 concentration was measured using Human IL-15 DuoSet ELISA kit (R&D Systems, Minneapolis, MN, USA) according to the manufacturer's protocol.
3
Quantification of Plasma Biomarkers
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Plasma IL-15, sMAdCAM-1 and LPS levels were quantified using Human IL-15 DuoSet ELISA kit (R&D Systems), Human MAdCAM-1 DuoSet ELISA kit (R&D Systems) and Human Lipopolysaccharides ELISA kit (MyBioSource) respectively, following manufacturers' recommendations. The values below the level of detection were assigned as zero.
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