Minocycline, Bovine Serum Albumin (lyophilized powder, ≥96% agarose gel electrophoresis), anhydrous ethanol (200 proof, anhydrous, ≥99.5%) crosslinker NHS-PEG-MAL-5000 and transferrin (human: minimum 98%) were purchased from Sigma Aldrich Saint Louis, MO, USA. HPLC Grade Ammonium acetate was purchased from VWR. HPLC-grade water, Methanol and Acetonitrile were purchased from Fisher Scientific. HPLC-grade glutaraldehyde (100%) was obtained from Alfa Aesar (Ward Hill, MA, USA). The Millipore Milli-Q Plus apparatus was used to procure ultrapure water. All other chemicals used in this study were of analytical grade. Traut’s reagent (2-Iminothiolane) and Ellman’s reagent were obtained from Pierce (Rockford, IL, USA). The PD-10 Columns SephadexTM G-25 M were from GE Healthcare. Lyophilizer, Centrifuge (Effendorf centrifuge 5810R, Thermo scientific sorvall RC 6+, Waltham, MA, USA), Zeta sizer (malvern), HPLC (Thermo Fischer), Sonicator and Rota evaporator (Rotaevapor Buchi, R-210) instruments were used in the study.
Pd 10 columns sephadex g 25 m
Manufactured by GE Healthcare
The PD-10 Columns SephadexTM G-25 M is a gel filtration chromatography column used for the desalting and buffer exchange of small molecules and proteins. It is designed to separate molecules based on their size and molecular weight.
Automatically generated - may contain errors
Lab products found in correlation
Acetonitrile, by Thermo Fisher Scientific (2 mentions)
Methanol, by Thermo Fisher Scientific (2 mentions)
High performance liquid chromatography (hplc), by Thermo Fisher Scientific (2 mentions)
Minocycline, by Merck Group (2 mentions)
Nhs peg mal 5000, by Merck Group (2 mentions)
Hplc grade glutaraldehyde, by Thermo Fisher Scientific (2 mentions)
Milli q plus apparatus, by Merck Group (2 mentions)
Hplc grade ammonium acetate, by Avantor (2 mentions)
Hplc grade water, by Thermo Fisher Scientific (2 mentions)
Bovine serum albumin (bsa), by Merck Group (2 mentions)
Transferrin, by Merck Group (2 mentions)
2 protocols using pd 10 columns sephadex g 25 m
1
Minocycline-Albumin Nanoparticle Synthesis
2
Minocycline-Based Drug Delivery Platform
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Minocycline, Bovine Serum Albumin (lyophilized powder, ≥96% agarose gel electrophoresis), anhydrous ethanol (200 proof, anhydrous, ≥99.5%) crosslinker NHS-PEG-MAL-5000 and transferrin (human: minimum 98%) were purchased from Sigma Aldrich U.S.A. HPLC Grade Ammonium acetate was purchased from VWR. HPLC grade water, Methanol and Acetonitrile were purchased from Fisher Scientific. HPLC-grade glutaraldehyde (100%) were obtained from Alfa Aesar (USA). The Millipore Milli-Q Plus apparatus was used to procure ultrapure water. Traut's reagent (2-Iminothiolane) and Ellman's reagent was obtained from Pierce (Rockford, USA). The PD-10 Columns SephadexTM G-25 M were from GE Healthcare. Lyophilizer, Centrifuge (Eppendorf Centrifuge 5810R, Thermo scientific sorvall RC 6+), Zeta sizer (malvern), HPLC (Thermo Fischer), Sonicator and Rota evaporator (Rotaevapor Buchi, R-210) instruments were used in the study.
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