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Cd1c biotin

Manufactured by Miltenyi Biotec
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The CD1c-biotin is a reagent that can be used to detect and isolate CD1c+ cells, which are a subset of dendritic cells. It is a biotinylated antibody that binds specifically to the CD1c surface marker.

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Lab products found in correlation

Anti biotin bead, by Miltenyi Biotec (2 mentions) Rosettesep monocyte enrichment kit, by STEMCELL (2 mentions) Automacs, by Miltenyi Biotec (2 mentions)

2 protocols using cd1c biotin

1

Isolation of Monocytes and Dendritic Cells

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APCs were enriched in blood from apheresed healthy donors by using a custom RosetteSep monocyte enrichment kit to deplete CD2, CD3, CD8, CD19, CD56, and CD66b (StemCell Technologies, custom kit catalog #15309) according to manufacturers protocols. Cells were then washed and cultured at 1×106 cells/ml at 37°C in R10. For cell sorting experiments, monocytes were further purified using positive selection CD14 microbeads. CD1c+ MDCs were purified from the negative fraction after CD14+ selection and CD19 microbead depletion, using CD1c-biotin followed by anti-biotin beads (Miltenyi). All separations were performed using an autoMACs (Miltenyi).
Thompson E.A., Darrah P.A., Foulds K.E., Hoffer E., Caffrey-Carr A., Norenstedt S., Perbeck L., Seder R.A., Kedl R.M, & Loré K. (2019). Monocytes Acquire the Ability to Prime Tissue-Resident T Cells via IL-10-Mediated TGF-β Release. Cell reports, 28(5), 1127-1135.e4.
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2

Isolation of Monocytes and Dendritic Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
APCs were enriched in blood from apheresed healthy donors by using a custom RosetteSep monocyte enrichment kit to deplete CD2, CD3, CD8, CD19, CD56, and CD66b (StemCell Technologies, custom kit catalog #15309) according to manufacturers protocols. Cells were then washed and cultured at 1×106 cells/ml at 37°C in R10. For cell sorting experiments, monocytes were further purified using positive selection CD14 microbeads. CD1c+ MDCs were purified from the negative fraction after CD14+ selection and CD19 microbead depletion, using CD1c-biotin followed by anti-biotin beads (Miltenyi). All separations were performed using an autoMACs (Miltenyi).
Thompson E.A., Darrah P.A., Foulds K.E., Hoffer E., Caffrey-Carr A., Norenstedt S., Perbeck L., Seder R.A., Kedl R.M, & Loré K. (2019). Monocytes Acquire the Ability to Prime Tissue-Resident T Cells via IL-10-Mediated TGF-β Release. Cell reports, 28(5), 1127-1135.e4.
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