D-amphetamine hemisulfate salt (Sigma-Aldrich, USA) was dissolved to a 0.3 mg/mL concentration in sterile saline. Competitive NMDAR antagonist DL-2-amino-5-phosphonopentanoic acid (AP5, Tocris Bioscience, GBR) was dissolved at 10 μg/μL in sterile saline.
Dl 2 amino 5 phosphonopentanoic acid ap5
Manufactured by Bio-Techne
Sourced in United States
DL-2-amino-5-phosphonopentanoic acid (AP5) is a chemical compound used in research laboratories. It functions as an N-methyl-D-aspartate (NMDA) receptor antagonist.
Automatically generated - may contain errors
Lab products found in correlation
D amphetamine hemisulfate salt, by Merck Group (1 mentions)
Picrotoxin, by Merck Group (1 mentions)
Borneol, by Merck Group (1 mentions)
Strychnine hydrochloride, by Merck Group (1 mentions)
6 cyano 7 nitroquinoxaline 2 3 dione cnqx, by Bio-Techne (1 mentions)
Tetrodotoxin citrate ttx, by Bio-Techne (1 mentions)
Glycine, by Merck Group (1 mentions)
2 protocols using dl 2 amino 5 phosphonopentanoic acid ap5
1
Amphetamine and NMDAR Antagonist Preparation
2
Electrophysiology of Borneol Responses
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Chemicals including borneol, picrotoxin, strychnine hydrochloride (strychnine), GABA, glycine, and ACSF compositions were purchased from Sigma-Aldrich (St. Louis, MO, USA), except for 6-cyano-7-nitro-quinoxaline-2,3-dione (CNQX), DL-2-amino-5-phosphonopentanoic acid (AP5), and tetrodotoxin citrate (TTX) by Tocris Bioscience (Avonmouth, Bristol, UK). Stocked drugs were dissolved in ACSF and tested at known concentrations.
In whole-cell voltage-clamp experiment, no less than 15 pA was considered to be responded by borneol to reduce possible error that may occur when measuring the currents. All data were presented as the mean ± standard error of the mean. A paired t-test was used to compare the mean values between the two groups. Statistical significance level was set as p < 0.05.
In whole-cell voltage-clamp experiment, no less than 15 pA was considered to be responded by borneol to reduce possible error that may occur when measuring the currents. All data were presented as the mean ± standard error of the mean. A paired t-test was used to compare the mean values between the two groups. Statistical significance level was set as p < 0.05.
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