The following antibodies were used: anti-GSK-3β (#9832), anti-K48-linked ubiquitin (#8081), NF-κB Pathway Sampler Kit (#9936), anti-TNF-R1 (#C25C1) and anti-RIP (#D94C12) (New England BioLabs, Frankfurt/Main, Germany), anti-Ubiquitin from DAKO (Hamburg, Germany), anti-pSer-31-NEMO, anti-TRAF6, anti-RIP1 and anti-β-actin (Abcam, Cambridge, UK); anti-p47 (Abnova, Heidelberg, Germany); phospho-specific anti-pSer-8-NEMO and anti-pSer-17-NEMO (Eurogentec, Köln, Germany); anti-FLAG M2 mouse (Sigma-Aldrich, Taufkirchen, Germany); anti-pSer-43-NEMO (Abgent, Heidelberg, Germany); anti-Tubulin and non-immune IgGs (Invitrogen, Darmstadt, Germany). Recombinant human TNFα was from Miltenyi (Bergisch Gladbach, Germany), GSK-3 inhibitors SB216730 and AR-A014418 from Calbiochem (La Jolla, CA, USA).
Anti rip1
Manufactured by Abcam
Sourced in Germany
Anti-RIP1 is a primary antibody that recognizes the receptor-interacting protein 1 (RIP1), a key mediator of cell death pathways. This antibody can be used for the detection and study of RIP1 in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti flag m2, by Merck Group (1 mentions)
Anti gsk 3β, by New England Biolabs (1 mentions)
Anti tubulin and nonimmune iggs, by Thermo Fisher Scientific (1 mentions)
Recombinant human tnfα, by Miltenyi Biotec (1 mentions)
Nf κb pathway sampler kit, by New England Biolabs (1 mentions)
Anti pser 43 nemo, by Abcepta (1 mentions)
Anti traf6, by Abcam (1 mentions)
Anti ubiquitin, by Agilent Technologies (1 mentions)
Anti β actin, by Abcam (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by ZSGB-BIO (1 mentions)
Anti rip3, by Abcam (1 mentions)
2 protocols using anti rip1
1
Signaling Pathway Antibody Panel
2
Immunohistochemistry of RIP1 and RIP3
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paraffin-embedded sections were routinely deparaffinized and rehydrated in xylene and different concentrations of ethanol. The sections were then incubated for 10 min with 3% H 2 O 2 to inhibit endogenous catalase. The sections underwent antigen retrieval with sodium citrate buffer for 15 min and were then incubated with anti-RIP1 (1:200, Abcam, Cambridge, Britain) and anti-RIP3 (1:500, Abcam, Cambridge, Britain) antibodies at room temperature for 1 h. The sections were rinsed with PBS and incubated with an appropriate amount of horseradish peroxidase-conjugated secondary antibodies (ZSGB-BIO, Beijing, China) at 37 °C for 30 min. Then, the sections were rinsed with PBS. DAB was used as the chromogen. The slides were detected under 400x magnification by light microscopy with a digital camera and imaging software. The positive cells were colored brown. The number of positive cells was counted in each image. The data were calculated as the percentage of immunohistochemistry-positive cells of the total number of cells per image.
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